{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T17:56:10Z","timestamp":1770832570815,"version":"3.50.1"},"reference-count":17,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2015,8,25]]},"abstract":"\n Background<\/jats:title>\n \n Diabetes is associated with coronary arteriolar endothelial dysfunction. We investigated the role of the small\/intermediate (\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n ) conductance of calcium\u2010activated potassium channels in diabetes\u2010related endothelial dysfunction.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Coronary arterioles (80 to 150\u00a0\u03bcm in diameter) were dissected from discarded right atrial tissues of diabetic (glycosylated hemoglobin = 9.6\u00b10.25) and nondiabetic patients (glycosylated hemoglobin 5.4\u00b10.12) during coronary artery bypass graft surgery (n=8\/group). In\u2010vitro relaxation response of precontracted arterioles was examined in the presence of the selective\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n activator\n NS<\/jats:styled-content>\n 309 and other vasodilatory agents. The channel density and membrane potential of diabetic and nondiabetic endothelial cells was measured by using the whole cell patch\u2010clamp technique. The protein expression and distribution of the\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n in the human myocardium and coronary arterioles was examined by Western blotting and immunohistochemistry. Our results indicate that diabetes significantly reduced the coronary arteriolar response to the\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n activator\n NS<\/jats:styled-content>\n 309 compared to the respective responses of nondiabetic vessels (\n P<\/jats:italic>\n <0.05 versus nondiabetes). The relaxation response of diabetic arterioles to\n NS<\/jats:styled-content>\n 309 was prevented by denudation of endothelium (\n P<\/jats:italic>\n =0.001 versus endothelium\u2010intact). Diabetes significantly decreased endothelial\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n currents and hyperpolarization induced by the\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n activator\n NS<\/jats:styled-content>\n 309 as compared with that of nondiabetics. There were no significant differences in the expression and distribution of\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n proteins in the coronary microvessels.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Diabetes is associated with inactivation of endothelial\n \n SK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n \/\n \n IK\n C<\/jats:sub>\n <\/jats:styled-content>\n a<\/jats:sub>\n channels, which may contribute to endothelial dysfunction in diabetic patients.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.115.002062","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T01:19:48Z","timestamp":1440465588000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Inactivation of Endothelial Small\/Intermediate Conductance of Calcium\u2010Activated Potassium Channels Contributes to Coronary Arteriolar Dysfunction in Diabetic Patients"],"prefix":"10.1161","volume":"4","author":[{"given":"Yuhong","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Cardiothoracic Surgery, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An","family":"Xie","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afshin","family":"Ehsan","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaurav","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Department of Medicine, Providence VA Medical Center, Alpert Medical School of Brown University, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Dudley","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank W.","family":"Sellke","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Feng","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Cardiovascular Research Center, Rhode Island Hospital, Providence, RI"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199807233390404"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703393"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.084590"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2012.04.009"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-010-9134-4"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706748"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjp.2008.108"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706417"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00040.2005"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2009.00132.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.755827"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2008.03.032"},{"key":"e_1_3_4_14_2","first-page":"724","article-title":"Impaired endothelium\u2010dependent coronary microvascular relaxation after cold potassium cardioplegia and reperfusion","volume":"55","author":"Sellke FW","year":"1993","unstructured":"Sellke FW, Shafique T, Schoen FJ, Weintraub RM. Impaired endothelium\u2010dependent coronary microvascular relaxation after cold potassium cardioplegia and reperfusion. Ann Thorac Surg. 1993;55:724\u2013728.","journal-title":"Ann Thorac Surg"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2010.04.005"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019318"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.172379"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.57.4.9"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.115.002062","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T10:42:58Z","timestamp":1715596978000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.115.002062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,8,25]]},"references-count":17,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2015,8,25]]}},"alternative-id":["10.1161\/JAHA.115.002062"],"URL":"https:\/\/doi.org\/10.1161\/jaha.115.002062","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,8,25]]},"assertion":[{"value":"2015-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e002062"}}