{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:48:00Z","timestamp":1790218080228,"version":"4.1.0"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2015,9,16]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Idiopathic dilated cardiomyopathy is one of the most common types of cardiomyopathy. It has been proposed that an increase in oxidative stress in heart failure leads to a decrease in nitric oxide signaling, leading to impaired nitroso\u2013redox signaling. To test this hypothesis, we investigated the occurrence of protein S\u2010nitrosylation (\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              ) and oxidation in biopsies from explanted dilated cardiomyopathy and nonfailing donor male and female human hearts.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Redox\u2010based resin\u2010assisted capture for oxidation and\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              proteomic analysis was used to measure protein oxidation and\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              , respectively. In addition, 2\u2010dimensional difference gel electrophoresis using maleimide sulfhydryl\u2010reactive fluors was used to identify the\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              proteins. Protein oxidation increased in dilated cardiomyopathy biopsies in comparison with those from healthy donors. Interestingly, we did not find a consistent decrease in\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              in failing hearts; we found that some proteins showed an increase in\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              and others showed a decrease, and there were sex\u2010specific differences in the response. We found 10 proteins with a significant decrease in\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              and 4 proteins with an increase in\n              <jats:styled-content style=\"fixed-case\">SNO<\/jats:styled-content>\n              in failing female hearts. Comparing nonfailing and failing male hearts, we found 9 proteins with a significant decrease and 12 proteins with a significant increase. We also found an increase in S\u2010glutathionylation of endothelial nitric oxide synthase in failing female versus male hearts, suggesting an increase in uncoupled nitric oxide synthase in female hearts.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p xml:lang=\"en\">These findings highlight the importance of nitroso\u2013redox signaling in both physiological and pathological conditions, suggesting a potential target to treat heart failure.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.115.002251","type":"journal-article","created":{"date-parts":[[2015,9,23]],"date-time":"2015-09-23T03:41:16Z","timestamp":1442979676000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Molecular Signature of Nitroso\u2013Redox Balance in Idiopathic Dilated Cardiomyopathies"],"prefix":"10.1161","volume":"4","author":[{"given":"Sara","family":"Menazza","sequence":"first","affiliation":[{"name":"Systems Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Aponte","sequence":"additional","affiliation":[{"name":"Proteomic Core Facility, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Sun","sequence":"additional","affiliation":[{"name":"Systems Biology Center, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Gucek","sequence":"additional","affiliation":[{"name":"Proteomic Core Facility, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, 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