{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T01:13:20Z","timestamp":1771636400431,"version":"3.50.1"},"reference-count":20,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2016,2,23]]},"abstract":"\n Background<\/jats:title>\n \n Transplantation of allogeneic mesenchymal stromal cells (\n MSC<\/jats:styled-content>\n s) is a promising treatment for heart failure. We have shown that epicardial placement of cell sheets markedly increases donor cell survival and augments therapeutic effects compared with the current methods. Although immune rejection of intramyocardially injected allogeneic\n MSC<\/jats:styled-content>\n s have been suggested, allogeneic\n MSC<\/jats:styled-content>\n s transplanted on the heart surface (virtual space) may undergo different courses. This study aimed to elucidate immunologic response against epicardially placed allogeneic\n MSC<\/jats:styled-content>\n s, rejection or acceptance of these cells, and their therapeutic effects for heart failure.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n At 4 weeks after coronary artery ligation, Lewis rats underwent epicardial placement of\n MSC<\/jats:styled-content>\n sheets from syngeneic Lewis or allogeneic Fischer 344 rats or sham treatment. At days 3 and 10 after treatment, similar ratios (\u224850% and 30%, respectively) of grafted\n MSC<\/jats:styled-content>\n s survived on the heart surface in both\n MSC<\/jats:styled-content>\n sheet groups. By day 28, survival of syngeneic\n MSC<\/jats:styled-content>\n s was substantially reduced (8.9%); survival of allogeneic\n MSC<\/jats:styled-content>\n s was more extensively reduced (0.2%), suggesting allorejection. Correspondingly, allogeneic\n MSC<\/jats:styled-content>\n s were found to have evoked an immunologic response, albeit low level, as characterized by accumulation of\n CD<\/jats:styled-content>\n 4\n +<\/jats:sup>\n T cells and upregulation of interleukin 6. Despite this alloimmune response, the allogeneic\n MSC<\/jats:styled-content>\n sheet achieved myocardial upregulation of reparative factors, enhanced repair of the failing myocardium, and improved cardiac function to the equivalent degree observed for the syngeneic\n MSC<\/jats:styled-content>\n sheet.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Allogeneic\n MSC<\/jats:styled-content>\n s placed on the heart surface evoked an immunologic response; however, this allowed sufficient early phase donor cell survival to induce equivalent therapeutic benefits to syngeneic\n MSC<\/jats:styled-content>\n s. Further development of this approach toward clinical application is warranted.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.115.002815","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T01:07:46Z","timestamp":1455930466000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Allogeneic Mesenchymal Stromal Cells Transplanted Onto the Heart Surface Achieve Therapeutic Myocardial Repair Despite Immunologic Responses in Rats"],"prefix":"10.1161","volume":"5","author":[{"given":"Nobuko","family":"Tano","sequence":"first","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Kaneko","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuki","family":"Ichihara","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiho","family":"Ikebe","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven R.","family":"Coppen","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Shiraishi","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunori","family":"Shintani","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenta","family":"Yashiro","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Warrens","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Suzuki","sequence":"additional","affiliation":[{"name":"William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, United 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