{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:41:36Z","timestamp":1778874096485,"version":"3.51.4"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2016,7,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Pigment epithelium\u2010derived factor (\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              ), which belongs to the noninhibitory serpin family, has shown the ability to stimulate several physiological processes, such as antiangiogenesis, anti\u2010inflammation, and antioxidation. In the present study, the effects of\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              on contractility and calcium handling of rat ventricular myocytes were investigated.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Adult Sprague\u2010Dawley rat models of acute myocardial infarction (\n              <jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content>\n              ) were surgically established.\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              \u2010lentivirus was delivered into the myocardium along and away from the infarction border to overexpress\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              . Video edge detection was used to measure myocyte shortening in\u00a0vitro. Intracellular Ca\n              <jats:sup>2+<\/jats:sup>\n              was measured in cells loaded with the Ca\n              <jats:sup>2+<\/jats:sup>\n              sensitive fluorescent indicator, Fura\u20102\u2010acetoxymethyl ester.\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              local overexpression enhanced cardiac functional reserve in\n              <jats:styled-content style=\"fixed-case\">AMI<\/jats:styled-content>\n              rats and reduced myocardial contracture bordering the infracted area. Exogenous\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              treatment (10\u00a0nmol\/L) caused a significant decrease in amplitudes of isoproterenol\u2010stimulated myocyte shortening, Ca\n              <jats:sup>2+<\/jats:sup>\n              transients, and caffeine\u2010evoked Ca\n              <jats:sup>2+<\/jats:sup>\n              transients in\u00a0vitro. We then tested a potential role for PEDF receptor\u2010mediated effects on upregulation of protein kinase C (\n              <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\n              ) and found evidence of signaling through the diacylglycerol\/\n              <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\n              \u03b1 pathway. We also confirmed that pretreatment of cardiomyocytes with\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              exhibited dephosphorylation of phospholamban at Ser\n              <jats:sup>16<\/jats:sup>\n              , which could be attenuated with\n              <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\n              inhibition.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              The results suggest that\n              <jats:styled-content style=\"fixed-case\">PEDF<\/jats:styled-content>\n              depresses myocyte contractility by suppressing phosphorylation of phospholamban and Ca\n              <jats:sup>2+<\/jats:sup>\n              transients in a\n              <jats:styled-content style=\"fixed-case\">PKC<\/jats:styled-content>\n              \u03b1\u2010dependent manner through its receptor, PEDF receptor, therefore improving cardiac functional reserve during AMI.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.115.003179","type":"journal-article","created":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T05:57:19Z","timestamp":1468475839000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Pigment Epithelium\u2010Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF\u2010R)"],"prefix":"10.1161","volume":"5","author":[{"given":"Peng","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Xu","sequence":"additional","affiliation":[{"name":"Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Research Center for Morphology, Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Yan","family":"Dong","sequence":"additional","affiliation":[{"name":"Research Center for Morphology, Xuzhou Medical University, Xuzhou, Jiangsu, 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