{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:01:15Z","timestamp":1772164875926,"version":"3.50.1"},"reference-count":70,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2016,8,8]]},"abstract":"\n Background<\/jats:title>\n \n Bioavailability of nitric oxide (\n NO<\/jats:styled-content>\n ) and hydrogen sulfide (H\n 2<\/jats:sub>\n S) is reduced in heart failure (\n HF<\/jats:styled-content>\n ). Recent studies suggest cross\u2010talk between\n NO<\/jats:styled-content>\n and H\n 2<\/jats:sub>\n S signaling. We previously reported that sodium nitrite (Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n ) ameliorates myocardial ischemia\u2010reperfusion injury and HF. Nuclear factor\u2010erythroid\u20102\u2010related factor 2 (Nrf2) regulates the antioxidant proteins expression and is upregulated by H\n 2<\/jats:sub>\n S. We examined the Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n effects on endogenous H\n 2<\/jats:sub>\n S bioavailability and Nrf2 activation in mice subjected to ischemia\u2010induced chronic heart failure (CHF).\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Mice underwent 60\u00a0minutes of left coronary artery occlusion and 4\u00a0weeks of reperfusion. Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n (165\u00a0\u03bcg\/kgic) or vehicle was administered at reperfusion and then in drinking water (100\u00a0mg\/L) for 4\u00a0weeks. Left ventricular (LV), ejection fraction (EF),\n LV<\/jats:styled-content>\n end diastolic (\n LVEDD<\/jats:styled-content>\n ) and systolic dimensions (\n LVESD<\/jats:styled-content>\n ) were determined at baseline and at 4\u00a0weeks of reperfusion. Myocardial tissue was analyzed for oxidative stress and respective gene\/protein\u2010related assays. We found that Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n therapy preserved LVEF, LVEDD and LVSD at 4\u00a0weeks during ischemia\u2010induced HF. Myocardial malondialdehyde and protein carbonyl content were significantly reduced in Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n \u2010treated mice as compared to vehicle, suggesting a reduction in oxidative stress. Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n therapy markedly increased expression of Cu,Zn\u2010superoxide dismutase, catalase, and glutathione peroxidase during 4\u00a0weeks of reperfusion. Furthermore, Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n upregulated the activity of Nrf2, as well as H\n 2<\/jats:sub>\n S\u2010producing enzymes, and ultimately increased H\n 2<\/jats:sub>\n S bioavailability in ischemia\u2010induced CHF in mice as compared with vehicle.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our results demonstrate that Na\n NO<\/jats:styled-content>\n 2<\/jats:sub>\n therapy significantly improves LV function via increasing H\n 2<\/jats:sub>\n S bioavailability, Nrf2 activation, and antioxidant defenses.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.116.003551","type":"journal-article","created":{"date-parts":[[2016,7,29]],"date-time":"2016-07-29T21:58:44Z","timestamp":1469829524000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":39,"title":["Nitrite Therapy Ameliorates Myocardial Dysfunction via H\n 2<\/sub>\n S and Nuclear Factor\u2010Erythroid 2\u2010Related Factor 2 (Nrf2)\u2010Dependent Signaling in Chronic Heart Failure"],"prefix":"10.1161","volume":"5","author":[{"given":"Erminia","family":"Donnarumma","sequence":"first","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shashi","family":"Bhushan","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica M.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Department of Surgery, Kurume University School of Medicine Kurume, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erinn L.","family":"Donnelly","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Lefer","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazi N.","family":"Islam","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2466"},{"key":"e_1_3_3_3_2","first-page":"230","article-title":"Nitric oxide and geriatrics: implications in diagnostics and treatment of the elderly","volume":"8","author":"Torregrossa AC","year":"2011","unstructured":"Torregrossa AC, Aranke M, Bryan NS. 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