{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T07:57:11Z","timestamp":1785225431574,"version":"3.55.0"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2016,6,13]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">The postnatal development of myofibrillar mechanics, a major determinant of heart function, is unknown in pediatric patients with tetralogy of Fallot and related structural heart defects. We therefore determined the mechanical properties of myofibrils isolated from right ventricular tissue samples from such patients in relation to the developmental changes of the isoforms expression pattern of key sarcomere proteins involved in the contractile process.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Tissue samples from the infundibulum obtained during surgery from 25 patients (age range 15\u00a0days to 11\u00a0years, median 7\u00a0months) were split into half for mechanical investigations and expression analysis of titin, myosin heavy and light chain 1, troponin\u2010T, and troponin\u2010I. Of these proteins, fetal isoforms of only myosin light chain 1 (\n              <jats:styled-content style=\"fixed-case\">ALC<\/jats:styled-content>\n              \u20101) and troponin\u2010I (ssTnI) were highly expressed in neonates, amounting to, respectively, 40% and 80%, while the other proteins had switched to the adult isoforms before or around birth.\n              <jats:styled-content style=\"fixed-case\">ALC<\/jats:styled-content>\n              \u20101 and ssTnI expression subsequently declined monoexponentially with a halftime of 4.3 and 5.8\u00a0months, respectively. Coincident with the expression of ssTnI, Ca\n              <jats:sup>2+<\/jats:sup>\n              sensitivity of contraction was high in neonates and subsequently declined in parallel with the decline in ssTnI expression. Passive tension positively correlated with Ca\n              <jats:sup>2+<\/jats:sup>\n              sensitivity but not with titin expression. Contraction kinetics, maximal Ca\n              <jats:sup>2+<\/jats:sup>\n              \u2010activated force, and the fast phase of the biphasic relaxation positively correlated with the expression of\n              <jats:styled-content style=\"fixed-case\">ALC<\/jats:styled-content>\n              \u20101.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">The developmental changes in myofibrillar biomechanics can be ascribed to fetal\u2010to\u2010adult isoform transition of key sarcomeric proteins, which evolves regardless of the specific congenital cardiac malformations in our pediatric patients.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.116.003699","type":"journal-article","created":{"date-parts":[[2016,6,29]],"date-time":"2016-06-29T17:54:51Z","timestamp":1467222891000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":15,"title":["Postnatal Development of Right Ventricular Myofibrillar Biomechanics in Relation to the Sarcomeric Protein Phenotype in Pediatric Patients with Conotruncal Heart Defects"],"prefix":"10.1161","volume":"5","author":[{"given":"Fatiha","family":"Elhamine","sequence":"first","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bogdan","family":"Iorga","sequence":"additional","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"},{"name":"Department of Physical Chemistry, University of Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Hunger","sequence":"additional","affiliation":[{"name":"Clinics for Anesthesiology and Surgical Intensive Care, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Eckhardt","sequence":"additional","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narayanswami","family":"Sreeram","sequence":"additional","affiliation":[{"name":"Pediatric Cardiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerardus","family":"Bennink","sequence":"additional","affiliation":[{"name":"Pediatric Heart Surgery, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konrad","family":"Brockmeier","sequence":"additional","affiliation":[{"name":"Pediatric Cardiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Pfitzer","sequence":"additional","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Stehle","sequence":"additional","affiliation":[{"name":"Institute of Vegetative Physiology, University of Cologne, K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","first-page":"H371","article-title":"Developmental changes in cardiac contractility in fetal and postnatal sheep: in\u00a0vitro and in\u00a0vivo","volume":"247","author":"Anderson PA","year":"1984","unstructured":"Anderson PA, Glick KL, Manring A, Crenshaw C Jr. Developmental changes in cardiac contractility in fetal and postnatal sheep: in\u00a0vitro and in\u00a0vivo. 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