{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T20:33:04Z","timestamp":1776371584692,"version":"3.51.2"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Large\u2010conductance calcium\u2010 and voltage\u2010activated potassium channels (\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channels) play important roles in the maintenance of vascular tone, and their dysregulation is associated with abnormal vascular relaxation and contraction. We tested the changes in\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channel properties in patients at different ages to assess the effects of hypertension and aging on the functional changes of\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channels.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Patch clamp was performed to detect the activities of\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channels in freshly isolated human mesenteric artery smooth muscle cells from younger patients (aged \u226445\u00a0years) without hypertension, older patients (aged \u226565\u00a0years) without hypertension, and older patients with hypertension. The expression of\n              <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n              and protein from\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channels was evaluated by reverse transcription polymerase chain reaction and Western blot analysis, respectively. Results showed that the whole\u2010cell current density, spontaneous transient outward current, and Ca\n              <jats:sup>2+<\/jats:sup>\n              sensitivity of the artery smooth muscle cells were significantly decreased in the older patients with hypertension; the decreases were insignificant in the older patients without hypertension, although a clear tendency to have spontaneous transient outward current was detected in these patients. The expression of both\n              <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n              and protein of\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              subunits \u03b1 and \u03b21 was significantly decreased in the older patients with hypertension but not in the older patients without hypertension compared with the younger patients without hypertension.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Our findings demonstrate for the first time that hypertension is an important factor for the pathological alteration of the properties of\n              <jats:styled-content style=\"fixed-case\">\n                BK\n                <jats:sub>C<\/jats:sub>\n              <\/jats:styled-content>\n              <jats:sub>a<\/jats:sub>\n              channels in human mesenteric artery smooth muscle cells, and aging itself may also be a factor in these changes in the cells.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.116.003913","type":"journal-article","created":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T02:29:06Z","timestamp":1473906546000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":21,"title":["Different Effects of Hypertension and Age on the Function of Large Conductance Calcium\u2010 and Voltage\u2010Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells"],"prefix":"10.1161","volume":"5","author":[{"given":"Jun","family":"Cheng","sequence":"first","affiliation":[{"name":"Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Mao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 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