{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T05:45:10Z","timestamp":1768801510191,"version":"3.49.0"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2017,11,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Following myocardial infarction (MI), peri\u2010infarct myocardial edema formation further impairs cardiac function. Extracellular RNA (eRNA) released from injured cells strongly increases vascular permeability. This study aimed to assess the role of eRNA in MI\u2010induced cardiac edema formation, infarct size, cardiac function, and survival after acute MI and to evaluate the therapeutic potential of ribonuclease 1 (RNase\u20101) treatment as an eRNA\u2010degrading intervention.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">C57BL\/6J mice were subjected to MI by permanent ligation of the left anterior descending coronary artery. Plasma eRNA levels were significantly increased compared with those in controls starting from 30\u00a0minutes after ligation. Systemic application of RNase\u20101, but not DNase, significantly reduced myocardial edema formation 24\u00a0hours after ligation compared with controls. Consequently, eRNA degradation by RNase\u20101 significantly improved the perfusion of collateral arteries in the border zone of the infarcted myocardium 24\u00a0hours after ligation of the left anterior descending coronary artery, as detected by micro\u2013computed tomography imaging. Although there was no significant difference in the area at risk, the area of vital myocardium was markedly larger in mice treated with RNase\u20101 compared with controls, as detected by Evans blue and 2,3,5\u2010triphenyltetrazolium chloride staining. The increase in viable myocardium was associated with significantly preserved left ventricular function, as assessed by echocardiography. Moreover, RNase\u20101 significantly improved 8\u2010week survival following MI.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">eRNA is an unrecognized permeability factor in\u00a0vivo, associated with myocardial edema formation after acute MI. RNase\u20101 counteracts eRNA\u2010induced edema formation and preserves perfusion of the infarction border zone, reducing infarct size and protecting cardiac function after MI.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.116.004541","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T00:20:26Z","timestamp":1498090826000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":29,"title":["Targeting of Extracellular RNA Reduces Edema Formation and Infarct Size and Improves Survival After Myocardial Infarction in Mice"],"prefix":"10.1161","volume":"6","author":[{"given":"Philipp","family":"Stieger","sequence":"first","affiliation":[{"name":"Department of Cardiology and Angiology, Otto\u2010von\u2010Guericke University Magdeburg, Magdeburg, 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