{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:10:46Z","timestamp":1770819046904,"version":"3.50.1"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2017,2,2]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Vinexin \u03b2 is a novel adaptor protein that regulates cellular adhesion, cytoskeletal reorganization, signal transduction, and transcription; however, the exact role that vinexin \u03b2 plays in atherosclerosis remains unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Immunoblot analysis showed that vinexin \u03b2 expression is upregulated in the atherosclerotic lesions of both patients with coronary heart disease and hyperlipemic apolipoprotein E\u2013deficient mice and is primarily localized in macrophages indicated by immunofluorescence staining. The high\u2010fat diet\u2013induced double\u2010knockout mice exhibited lower aortic plaque burdens than apolipoprotein E\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              littermates and decreased macrophage content. Vinexin \u03b2 deficiency improved plaque stability by attenuating lipid accumulation and increasing smooth muscle cell content and collagen. Moreover, the bone marrow transplant experiment demonstrated that vinexin \u03b2 deficiency exerts atheroprotective effects in hematopoietic cells. Consistent with these changes, the\n              <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n              expression of proinflammatory cytokines were downregulated in vinexin \u03b2\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              apolipoprotein E\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice, whereas the anti\u2010inflammatory M2 macrophage markers were upregulated. The immunohistochemical staining and in\u00a0vitro experiments showed that deficiency of vinexin \u03b2 inhibited the accumulation of monocytes and the migration of macrophages induced by tumor necrosis factor \u03b1\u2013stimulated human umbilical vein endothelial cells as well as macrophage proliferation. Finally, the inhibitory effects exerted by vinexin \u03b2 deficiency on foam cell formation, nuclear factor \u03baB activation, and inflammatory cytokine expression were largely reversed by constitutive Akt activation, whereas the increased expression of the nuclear factor \u03baB subset promoted by adenoviral vinexin \u03b2 was dramatically suppressed by inhibition of\n              <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Vinexin \u03b2 deficiency attenuates atherogenesis primarily by suppressing vascular inflammation and inactivating Akt\u2013nuclear factor \u03baB signaling. Our data suggest that vinexin \u03b2 could be a therapeutic target for the treatment of atherosclerosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.116.004585","type":"journal-article","created":{"date-parts":[[2017,2,17]],"date-time":"2017-02-17T02:01:02Z","timestamp":1487296862000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["Vinexin \u03b2 Ablation Inhibits Atherosclerosis in Apolipoprotein E\u2013Deficient Mice by Inactivating the Akt\u2013Nuclear Factor \u03baB Inflammatory Axis"],"prefix":"10.1161","volume":"6","author":[{"given":"Hongjing","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"Cardiovascular Research Institute, Wuhan University, Wuhan, China"},{"name":"Hubei Key Laboratory of Cardiology, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Lin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junhong","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng\u2010Lin","family":"Chao","sequence":"additional","affiliation":[{"name":"Key Laboratory of CVD, Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Yong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi\u2010Gang","family":"She","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zan","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Science, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongliang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, China"},{"name":"The Institute of Model Animals of Wuhan University, Wuhan, China"},{"name":"Medical Research Institute, School of Medicine, Wuhan University, Wuhan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e3182009701"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.192667"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901143400207"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01323"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302721"},{"key":"e_1_3_5_7_2","first-page":"1356","article-title":"A novel adaptor protein family regulating cytoskeletal organization and signal transduction\u2014Vinexin, CAP\/ponsin, ArgBP2","volume":"74","author":"Kioka N","year":"2002","unstructured":"Kioka N. [A novel adaptor protein family regulating cytoskeletal organization and signal transduction\u2014Vinexin, CAP\/ponsin, ArgBP2]. Seikagaku. 2002;74:1356\u20131360.","journal-title":"Seikagaku"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.1.59"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1247\/csf.27.1"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.50.35933"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M501344200"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2006.00995.x"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0338-0"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20140648"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1749"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.92.5.1355"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.12991"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11432"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10592"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20665"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000160340.72641.87"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3258"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2675"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110572200"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0329fje"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208296"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5262.685"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.14.1.133"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90362-G"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/hc4501.098952"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1411543"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.04.005"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3520"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604260103"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2016.03.021"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0706823"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvv130"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2010.11.001"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1160\/TH14-06-0509"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2423"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118598"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(01)00546-9"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401060101"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021909-135846"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.621"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-262097"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2009.00900.x"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.116.004585","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T10:54:57Z","timestamp":1715597697000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.116.004585"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,2]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2,2]]}},"alternative-id":["10.1161\/JAHA.116.004585"],"URL":"https:\/\/doi.org\/10.1161\/jaha.116.004585","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,2]]},"assertion":[{"value":"2016-08-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e004585"}}