{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T05:30:56Z","timestamp":1771997456019,"version":"3.50.1"},"reference-count":52,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"12","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2017,12,2]]},"abstract":"\n Background<\/jats:title>\n \n Congestive heart failure (\n CHF<\/jats:styled-content>\n ) is a common cardiovascular disease that is often accompanied by ventricular arrhythmias. The decrease of the slow component of the delayed rectifier potassium current (\n \n I\n K<\/jats:sub>\n <\/jats:styled-content>\n s<\/jats:sub>\n ) in\n CHF<\/jats:styled-content>\n leads to action potential (\n AP<\/jats:styled-content>\n ) prolongation, and the\n \n I\n K<\/jats:sub>\n <\/jats:styled-content>\n s<\/jats:sub>\n is an important contributor to the development of ventricular arrhythmias. However, the molecular mechanisms underlying ventricular arrhythmias are still unknown.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Kcna2 and Kcna2 antisense RNA (Kcna2\n AS)<\/jats:styled-content>\n transcript expression was measured in rat cardiac tissues using quantitative real\u2010time reverse transcription\u2013polymerase chain reaction and Western blotting. There was a 43% reduction in Kcna2\n mRNA<\/jats:styled-content>\n in the left ventricular myocardium of rats with\n CHF<\/jats:styled-content>\n . Kcna2 knockdown in the heart decreased the I\n Ks<\/jats:sub>\n and prolonged\n AP<\/jats:styled-content>\n s in cardiomyocytes, consistent with the changes observed in heart failure. Conversely, Kcna2 overexpression in the heart significantly attenuated the\n CHF<\/jats:styled-content>\n \u2010induced decreases in the I\n Ks<\/jats:sub>\n ,\n AP<\/jats:styled-content>\n prolongation, and ventricular arrhythmias. Kcna2\n AS<\/jats:styled-content>\n was upregulated \u22481.7\u2010fold in rats with\n CHF<\/jats:styled-content>\n and with phenylephrine\u2010induced cardiomyocyte hypertrophy. Kcna2\n AS<\/jats:styled-content>\n inhibition increased the\n CHF<\/jats:styled-content>\n \u2010induced downregulation of Kcna2. Consequently, Kcna2 AS mitigated the decrease in the I\n Ks<\/jats:sub>\n and the prolongation of\n AP<\/jats:styled-content>\n s in\u00a0vivo and in\u00a0vitro and reduced ventricular arrhythmias, as detected using electrocardiography.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Ventricular Kcna2\n AS<\/jats:styled-content>\n expression increases in rats with\n CHF<\/jats:styled-content>\n and contributes to reduced\n \n I\n K<\/jats:sub>\n <\/jats:styled-content>\n s<\/jats:sub>\n , prolonged\n AP<\/jats:styled-content>\n s, and the occurrence of ventricular arrhythmias by silencing Kcna2. Thus, Kcna2\n AS<\/jats:styled-content>\n may be a new target for the prevention and treatment of ventricular arrhythmias in patients with\n CHF<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.117.005965","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T01:11:58Z","timestamp":1513818718000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":42,"title":["Long Noncoding RNA Kcna2 Antisense RNA Contributes to Ventricular Arrhythmias via Silencing Kcna2 in Rats With Congestive Heart Failure"],"prefix":"10.1161","volume":"6","author":[{"given":"Qing\u2010Qing","family":"Long","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Geriatrics, Sir Run Run Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya\u2010Fei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Emergency and Intensive Care, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u2010Jie","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing\u2010Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao\u2010Yu","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ze\u2010Mu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lian\u2010Sheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/NCI.0b013e3181b82379"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S73216"},{"key":"e_1_3_5_4_2","first-page":"I50","article-title":"Lack of relation between ventricular arrhythmias and sudden death in patients with chronic heart failure","volume":"85","author":"Packer M","year":"1992","unstructured":"Packer M. 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