{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T12:50:55Z","timestamp":1776862255387,"version":"3.51.2"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2018,4,17]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Subarachnoid hemorrhage is a serious clinical condition that impairs local cerebral blood flow perfusion and consequently initiates neuronal dysfunction. Pressure\u2010sensitive myogenic vasomotor regulation is an important mechanism involved in the regulation of cerebral blood flow. We hypothesized that extravascular hemolyzed blood enhances arteriolar myogenic constriction, which in\u00a0vivo may contribute to the reduction of local cerebral blood flow after subarachnoid hemorrhage.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Arterioles isolated from the middle cerebral artery (\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              arterioles) of mice were cannulated in a perfusion chamber. Arteriolar diameters in response to step increases in intraluminal pressure (20\u2013120\u00a0mm\u00a0Hg) were measured in various experimental conditions. In response to increases in intraluminal pressure, all\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              arterioles exhibited myogenic vasoconstrictions. Compared with controls, the pressure\u2010induced constriction was significantly enhanced in arterioles (in\u00a0vitro) exposed to extravascular hemolyzed blood or different concentrations of extracellular erythrocyte lysate (1%, 10%, and 20%) for different exposure durations (1\u20136\u00a0hours). The magnitude of enhancement was proportional to the lysate concentration and exposure duration. In in\u00a0vivo experiments, 10\u00a0\u03bcL of autologous blood lysate were injected into the mouse subarachnoid space on the surface of the left\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              . Two hours later,\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              arterioles were isolated and left\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              arterioles displayed enhanced myogenic responses compared with the right\n              <jats:styled-content style=\"fixed-case\">MCA<\/jats:styled-content>\n              . The enhanced myogenic response was prevented by scavenge of superoxide in both in\u00a0vitro and in\u00a0vivo experiments.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Extravascular hemolyzed blood, perhaps by promoting vascular production of superoxide, augments myogenic constriction of cerebral arterioles, which plays a crucial role in the subarachnoid hemorrhage\u2013induced cerebral ischemia.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.118.008623","type":"journal-article","created":{"date-parts":[[2018,4,13]],"date-time":"2018-04-13T11:50:13Z","timestamp":1523620213000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Extravascular Blood Augments Myogenic Constriction of Cerebral Arterioles: Implications for Hemorrhage\u2010Induced Vasospasm"],"prefix":"10.1161","volume":"7","author":[{"given":"Wensheng","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"},{"name":"Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharath","family":"Kandhi","sequence":"additional","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"},{"name":"Department of GI Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akos","family":"Koller","sequence":"additional","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"},{"name":"Institute of Natural Sciences, Sportgenetics and Sportgerontology Res. Group, University of Physical Education, Budapest, Hungary"},{"name":"Department of Neurosurgery, Medical School, University of Pecs, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physiology, New York Medical College, Valhalla, NY"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60153-6"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.531020"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000338747"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/hs0901.095677"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068498"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.66.1.8"},{"key":"e_1_3_3_8_2","first-page":"H1486","article-title":"Characteristics and origin of myogenic response in isolated mesenteric arterioles","volume":"263","author":"Sun D","year":"1992","unstructured":"Sun D, Messina EJ, Kaley G, Koller A. Characteristics and origin of myogenic response in isolated mesenteric arterioles. Am J Physiol. 1992;263:H1486\u2013H1491.","journal-title":"Am J Physiol"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00230.2009"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00920.2013"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.115.006353"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/124.2.249"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2013.173"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocn.2014.05.025"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000437112"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)93432-O"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600471"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2011.154"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200003000-00018"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2015.4365"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00114.2016"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00786.2014"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00187.2015"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.170472"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1998.89.5.0748"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.9.960"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000079165.84309.4D"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00321.2014"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.115.05274"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00267.2016"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.28.7.1396"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.111.000188"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.118.008623","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:20:06Z","timestamp":1715599206000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.118.008623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,17]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,4,17]]}},"alternative-id":["10.1161\/JAHA.118.008623"],"URL":"https:\/\/doi.org\/10.1161\/jaha.118.008623","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,17]]},"assertion":[{"value":"2018-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e008623"}}