{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T23:24:40Z","timestamp":1794007480522,"version":"4.8.0"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2018,8,7]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Exosomes are membranous vesicles generated by almost all cells. Recent studies demonstrated that mesenchymal stem cell\u2013derived exosomes possessed many effects, including antiapoptosis, anti\u2010inflammatory effects, stimulation of angiogenesis, anticardiac remodeling, and recovery of cardiac function on cardiovascular diseases. However, targeting of exosomes to recipient cells precisely in\u00a0vivo still remains a problem. Ligand fragments or homing peptides discovered by phage display and in\u00a0vivo biopanning methods fused to the enriched molecules on the external part of exosomes have been exploited to improve the ability of exosomes to target specific tissues or organs carrying cognate receptors. Herein, we briefly elucidated how to improve targeting ability of exosomes to ischemic myocardium.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      We used technology of molecular cloning and lentivirus packaging to engineer exosomal enriched membrane protein (Lamp2b) fused with ischemic myocardium\u2010targeting peptide CSTSMLKAC (IMTP). In vitro results showed that IMTP\u2010exosomes could be internalized by hypoxia\u2010injured H9C2 cells more efficiently than blank\u2010exosomes. Compared with blank\u2010exosomes, IMTP\u2010exosomes were observed to be increasingly accumulated in ischemic heart area (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.05). Meanwhile, attenuated inflammation and apoptosis, reduced fibrosis, enhanced vasculogenesis, and cardiac function were detected by mesenchymal stem cell\u2013derived IMTP\u2010exosome treatment in ischemic heart area.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Our research concludes that exosomes engineered by IMTP can specially target ischemic myocardium, and mesenchymal stem cell\u2013derived IMTP\u2010exosomes exert enhanced therapeutic effects on acute myocardial infarction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.118.008737","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T02:43:15Z","timestamp":1537065795000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":390,"title":["Engineered Exosomes 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