{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:05:23Z","timestamp":1791871523046,"version":"4.3.4"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,1,8]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              The poor viability of transplanted mesenchymal stem cells (MSCs) hampers their therapeutic efficacy for ischemic heart disease. Micro\n              <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n              s are involved in regulation of\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              survival and function. The present study was designed to investigate the molecular effects of mi\n              <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n              \u201015a\/15b on\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              survival, focusing on the role of vascular endothelial growth factor receptor 2.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              We first harvested donor luc(Luciferase)\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s (5\u00d710\n              <jats:sup>5<\/jats:sup>\n              ) isolated from the luciferase transgenic mice with\n              <jats:styled-content style=\"fixed-case\">FVB<\/jats:styled-content>\n              background. Luc\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s were transfected with miR\u201015a\/15b mimics or inhibitors and cultured under oxygen glucose deprivation condition for 12\u00a0hours to mimics the harsh microenvironment in infarcted heart; they were subjected to MTT (3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide?Thiazolyl Blue Tetrazolium Bromide) assay, bioluminescence imaging, quantitative reverse transcription\u2013polymerase chain reaction, transferase\u2010mediated deoxyuridine triphosphate\u2013digoxigenin nick\u2010end labeling assay, and flow cytometry. Furthermore, the levels of vascular endothelial growth factor receptor 2, protein kinase B, p(Phosphorylate)\u2010protein kinase B, Bcl\u20102, Bax, and caspase\u20103 proteins were available by Western blotting assay. In vivo, acute myocardial infarction was induced in 24 mice by coronary ligation, with subsequent receipt of Luc\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s, Luc\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s+miR\u201015a\/15b inhibitors, or\n              <jats:styled-content style=\"fixed-case\">PBS<\/jats:styled-content>\n              treatment. The therapeutic procedure and treatment effects were tracked and assessed using bioluminescence imaging and echocardiographic measurement. Next, ex\u00a0vivo imaging and immunohistochemistry were conducted to verify the distribution of\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s. We demonstrated that miR\u201015a\/15b targeted vascular endothelial growth factor receptor 2 to modulate\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              survival, possibly via phosphatidylinositol 3\u2010kinase\/protein kinase B signaling pathway, which was proved by bioluminescence imaging, immunohistochemistry analysis, and echocardiographic measurement.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Luc\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s could be followed dynamically in\u00a0vitro and in\u00a0vivo by bioluminescence imaging, and the role of mi\n              <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n              \u201015a\/b could be inferred from the loss of signals from luc\u2010\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              s. This finding may have practical clinical implications in mi\n              <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>\n              \u201015a\/15b\u2013modified\n              <jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>\n              transplantation in treating myocardial infarction.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.118.010157","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T10:18:54Z","timestamp":1546942734000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":12,"title":["miR\u201015a\/15b Cluster Modulates Survival of Mesenchymal Stem Cells to Improve Its Therapeutic Efficacy of Myocardial Infarction"],"prefix":"10.1161","volume":"8","author":[{"given":"Yingfeng","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Cardiology The 2nd Hospital of Harbin Medical University Nangang District Harbin China"},{"name":"The Key Laboratory of Myocardial Ischemia Chinese Ministry of Education Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Huadong sanatorium Wuxi City Jiangsu Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology The Third Hospital of Harbin Medical University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Radiology The Fourth Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Cardiology The 2nd Hospital of Harbin Medical University Nangang District Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youbin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology The 2nd Hospital of Harbin Medical University Nangang District Harbin China"},{"name":"The Key Laboratory of Myocardial Ischemia Chinese Ministry of Education Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenguang","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Basic Medical Science Harbin Medical University\u2013Daqing Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Hepatosplenic Surgery Department of General Surgery The First Affiliated Hospital of Harbin Medical University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhua","family":"Fan","sequence":"additional","affiliation":[{"name":"College of Pharmacy Harbin Medical University\u2013Daqing Daqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cardiology The 2nd Hospital of Harbin Medical University Nangang District Harbin China"},{"name":"The Key Laboratory of Myocardial Ischemia Chinese Ministry of Education Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2014.47881"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/clc.22367"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0676-1"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4285938"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000480730"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-017-0136-x"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1755-5922.12305"},{"key":"e_1_3_1_9_2","article-title":"Mesenchymal stem cell therapy: a promising cell based therapy for treatment of myocardial infraction","volume":"19","author":"El Sayed Shafei A","year":"2017","unstructured":"El Sayed Shafei A, Ali MA, Ghanem HG, Shehata AI, Abdelgawad AA, Handal HR, Talaat KA, Ashaal AE, El\u2010Shal AS. 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