{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:46:06Z","timestamp":1790023566585,"version":"4.0.1"},"reference-count":58,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,3,19]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Endothelial insulin resistance is insulin\u2010insensitivity in the vascular endothelium and can be observed in experimental models. This study aimed to investigate endothelial insulin resistance in patients with suspected coronary artery disease. To this end, a novel method of obtaining freshly isolated arterial endothelial cells from a radial catheter sheath was developed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Freshly isolated arterial endothelial cells were retrieved from catheter sheaths placed in radial arteries for coronary angiography (n=69, patient age 64\u00b112\u00a0years). The endothelial cells were divided into groups for incubation with or without insulin, vascular endothelial growth factor, or acetylcholine. The intensity of phosphorylated endothelial nitric oxide synthase at Ser1177 (p\u2010\n              <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>\n              ) was quantified by immunofluorescence microscopy. The percentage increase of insulin\u2010induced phosphorylated endothelial nitric oxide synthase correlated negatively with derivatives of reactive oxygen metabolites, an oxidative stress test (\n              <jats:italic>r<\/jats:italic>\n              =\u22120.348, n=53,\n              <jats:italic>P<\/jats:italic>\n              =0.011), E\/E\u2032, an index of left ventricular diastolic dysfunction in Doppler echocardiography (\u03c1=\u22120.374, n=49,\n              <jats:italic>P<\/jats:italic>\n              =0.008), and log\u2010transformed brain natriuretic peptide (\n              <jats:italic>r<\/jats:italic>\n              =\u22120.266, n=62,\n              <jats:italic>P<\/jats:italic>\n              =0.037). Furthermore, percentage increase of insulin\u2010induced p\u2010\n              <jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content>\n              was an independent factor for the cardio\u2010ankle vascular index (standardized coefficient \u03b2=\u22120.293, n=42,\n              <jats:italic>P<\/jats:italic>\n              =0.021) in the multivariate regression analysis of adaptive least absolute shrinkage and selection operator.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Our results suggested that endothelial insulin resistance is associated with oxidative stress, left ventricular diastolic dysfunction, heart failure, and arterial stiffness.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.118.010816","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T09:04:02Z","timestamp":1552986242000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["Endothelial Insulin Resistance of Freshly Isolated Arterial Endothelial Cells From Radial Sheaths in Patients With Suspected Coronary Artery Disease"],"prefix":"10.1161","volume":"8","author":[{"given":"Nobuyuki","family":"Masaki","sequence":"first","affiliation":[{"name":"Department of Intensive Care Medicine National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Ido","sequence":"additional","affiliation":[{"name":"Department of Cardiology National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiyuki","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery Keio University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhei","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Cardiology National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Toya","sequence":"additional","affiliation":[{"name":"Department of Cardiology National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonpei","family":"Takase","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Medicine National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi M.","family":"Hamburg","sequence":"additional","affiliation":[{"name":"The Whitaker Cardiovascular Institute Department of Medicine Boston University School of Medicine Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Cardiology National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.306206"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.198028"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-012-9229-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/21218"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M103702200"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.172916"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704956"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.08.011"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12711"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1098\/rsif.2013.0852"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.127514"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.115.306578"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00340.2016"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.5551\/jat.7716"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10157-006-0453-4"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1163\/156856700X00769"},{"key":"e_1_3_2_18_2","first-page":"127","article-title":"A simple test to monitor oxidative stress","volume":"18","author":"Cesarone MR","year":"1999","unstructured":"Cesarone MR, Belcaro G, Carratelli M, Cornelli U, De Sanctis MT, Incandela L, Barsotti A, Terranova R, Nicolaides A. A simple test to monitor oxidative stress. Int Angiol. 1999;18:127\u2013130.","journal-title":"Int Angiol"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.20.7.1087"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2018.00064"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-15-1257"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-19425-7_4"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.07.003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000255954.80025.34"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000255955.75119.1a"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1536\/ihj.16-112"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hfc.2012.06.005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01058"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000242791.28953.4c"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000126501.34994.96"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2013.00161"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1538-7836.2003.00197.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-016-0443-0"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.10.079"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.1.168"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-247148"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0797-1"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00407.2012"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(92)90271-N"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9149(92)90710-G"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.11.082"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000153349.77489.CF"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-10-0910"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10741-015-9471-1"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000160548.78317.29"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000151373.33830.41"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000138068.03893.40"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000239206.64270.5f"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.12.027"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2015.05.008"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.05.004"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.07.225"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20110621"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0186311"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00142.2011"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.31.10.2487"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.2337\/db05-1147"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2007.03.023"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2007"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.118.010816","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T10:57:57Z","timestamp":1715597877000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.118.010816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,19]]},"references-count":58,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,3,19]]}},"alternative-id":["10.1161\/JAHA.118.010816"],"URL":"https:\/\/doi.org\/10.1161\/jaha.118.010816","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,19]]},"assertion":[{"value":"2018-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-02-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e010816"}}