{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:25:42Z","timestamp":1758403542907,"version":"3.41.2"},"reference-count":56,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,6,4]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Pathophysiological mechanisms that connect obesity to cardiovascular disease are incompletely understood. FSP27 (Fat\u2010specific protein 27) is a lipid droplet\u2010associated protein that regulates lipolysis and insulin sensitivity in adipocytes. We unexpectedly discovered extensive\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 expression in human endothelial cells that is downregulated in association with visceral obesity. We sought to examine the functional role of\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 in the control of vascular phenotype.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              We biopsied paired subcutaneous and visceral fat depots from 61 obese individuals (body mass index 44\u00b18\u00a0kg\/m\n              <jats:sup>2<\/jats:sup>\n              , age 48\u00b14\u00a0years) during planned bariatric surgery. We characterized depot\u2010specific\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 expression in relation to adipose tissue microvascular insulin resistance, endothelial function and angiogenesis, and examined differential effects of\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 modification on vascular function. We observed markedly reduced vasodilator and angiogenic capacity of microvessels isolated from the visceral compared with subcutaneous adipose depots. Recombinant\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 and\/or adenoviral\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 overexpression in human tissue increased endothelial nitric oxide synthase phosphorylation and nitric oxide production, and rescued vasomotor and angiogenic dysfunction (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05), while si\n              <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n              \u2010mediated\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 knockdown had opposite effects. Mechanistically, we observed that\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 interacts with vascular endothelial growth factor\u2010A and exerts robust regulatory control over its expression. Lastly, in a subset of subjects followed longitudinally for 12\u00b13\u00a0months after their bariatric surgery, 30% weight loss improved metabolic parameters and increased angiogenic capacity that correlated positively with increased\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 expression (\n              <jats:italic>r<\/jats:italic>\n              =0.79,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Our data strongly support a key role and functional significance of\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 as a critical endogenous modulator of human microvascular function that has not been previously described.\n              <jats:styled-content style=\"fixed-case\">FSP<\/jats:styled-content>\n              27 may serve as a previously unrecognized regulator of arteriolar vasomotor capacity and angiogenesis which are pivotal in the pathogenesis of cardiometabolic diseases linked to obesity.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.118.011431","type":"journal-article","created":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T09:14:21Z","timestamp":1559380461000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Fat\u2010Specific Protein 27 Regulation of Vascular Function in Human Obesity"],"prefix":"10.1161","volume":"8","author":[{"given":"Shakun","family":"Karki","sequence":"first","affiliation":[{"name":"Evans Department of Medicine and Whitaker Cardiovascular Institute Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa G.","family":"Farb","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine and Whitaker Cardiovascular Institute Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishva M.","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute Ohio University Athens OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sukanta","family":"Jash","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute Ohio University Athens OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaina J.","family":"Zizza","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine and Whitaker Cardiovascular Institute Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald T.","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of General Surgery Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Carmine","sequence":"additional","affiliation":[{"name":"Department of General Surgery Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cullen O.","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of General Surgery Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luise I.","family":"Pernar","sequence":"additional","affiliation":[{"name":"Department of General Surgery Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline M.","family":"Apovian","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine and Whitaker Cardiovascular Institute Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishwajeet","family":"Puri","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Diabetes Institute Ohio University Athens OH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noyan","family":"Gokce","sequence":"additional","affiliation":[{"name":"Evans Department of Medicine and Whitaker Cardiovascular Institute Boston University School of Medicine Boston MA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","first-page":"1","article-title":"Prevalence of obesity among adults and youth: United States, 2011\u20132014","volume":"219","author":"Ogden CL","year":"2015","unstructured":"Ogden CL, Carroll MD, Fryar CD, Flegal KM. 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