{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:27:32Z","timestamp":1792009652877,"version":"4.4.0"},"reference-count":34,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,5,7]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Oligodendrocyte precursor cells (\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s) regulate neuronal, glial, and vascular systems in diverse ways and display phenotypic heterogeneity beyond their established role as a reservoir for mature oligodendrocytes. However, the detailed phenotypic changes of\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s after cerebral ischemia remain largely unknown. Here, we aimed to investigate the roles of reactive\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s in the ischemic brain.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              The behavior of\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s was evaluated in a mouse model of ischemic stroke produced by transient middle cerebral artery occlusion in\u00a0vivo. For in\u00a0vitro experiments, the phenotypic change of\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s after oxygen glucose derivation was examined using a primary rat\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              culture. Furthermore, the therapeutic potential of hypoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s was evaluated in a mouse model of middle cerebral artery occlusion in\u00a0vivo. Perivascular\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s in the cerebral cortex were increased alongside poststroke angiogenesis in a mouse model of middle cerebral artery occlusion. In vitro\n              <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n              \u2010seq analysis revealed that primary cultured\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s increased the gene expression of numerous pro\u2010angiogenic factors after oxygen glucose derivation. Hypoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s secreted a greater amount of pro\u2010angiogenic factors, such as vascular endothelial growth factor\u00a0and angiopoietin\u20101, compared with normoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s. Hypoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              \u2010derived conditioned media increased the viability and tube formation of endothelial cells. In vivo studies also demonstrated that 5 consecutive daily treatments with hypoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              \u2010conditioned media, beginning 2\u00a0days after middle cerebral artery occlusion, facilitated poststroke angiogenesis, alleviated infarct volume, and improved functional disabilities.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Following cerebral ischemia, the phenotype of\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s in the cerebral cortex shifts from the parenchymal subtype to the perivascular subtype, which can promote angiogenesis. The optimal use of hypoxic\n              <jats:styled-content style=\"fixed-case\">OPC<\/jats:styled-content>\n              s secretome would provide a novel therapeutic option for stroke.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.118.011824","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T09:15:18Z","timestamp":1556183718000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["Role of Perivascular Oligodendrocyte Precursor Cells in Angiogenesis After Brain Ischemia"],"prefix":"10.1161","volume":"8","author":[{"given":"Natsue","family":"Kishida","sequence":"first","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"},{"name":"Department of Neurosurgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takakuni","family":"Maki","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Takagi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine Kyoto University Kyoto Japan"},{"name":"Department of Neurosurgery Graduate School of Medicine Tokushima University Tokushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisanori","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Ayaki","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Noro","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology KAN Research Institute, Inc. Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Ono","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology KAN Research Institute, Inc. Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroharu","family":"Kataoka","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumichi","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eng H.","family":"Lo","sequence":"additional","affiliation":[{"name":"Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Arai","sequence":"additional","affiliation":[{"name":"Departments of Radiology and Neurology Massachusetts General Hospital and Harvard Medical School Charlestown Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Neurology Graduate School of Medicine Kyoto University Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.07.002"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2226"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1871527311312030004"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf6463"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2016.159"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4222"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2017.09.015"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2015.04.047"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103174"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1592-15.2015"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199607000-00010"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4188"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.26.4.627"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.018234"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2015.05.001"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2017.12.001"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.117.019346"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1972"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0483-6"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2015.02.042"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.052"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm781"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad3839"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.05.019"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2015.09.010"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.01.006"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2001-12.2013"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2831-08.2008"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2220"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2810-14.2015"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12975-017-0601-z"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.110.603399"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.118.011824","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:36:46Z","timestamp":1715600206000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.118.011824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,7]]},"references-count":34,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,5,7]]}},"alternative-id":["10.1161\/JAHA.118.011824"],"URL":"https:\/\/doi.org\/10.1161\/jaha.118.011824","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,5,7]]},"assertion":[{"value":"2019-01-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e011824"}}