{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:42:31Z","timestamp":1773301351023,"version":"3.50.1"},"reference-count":72,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,5,7]]},"abstract":"\n Background<\/jats:title>\n \n The redox\u2010sensitive chaperone\n DJ<\/jats:styled-content>\n \u20101 and uncoupling protein 2 are protective against mitochondrial oxidative stress. We previously reported that renal\u2010selective depletion and germline deletion of\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n increases blood pressure in mice. This study aimed to determine the mechanisms involved in the oxidative stress\u2013mediated hypertension in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n There were no differences in sodium excretion, renal renin expression, renal\n NADPH<\/jats:styled-content>\n oxidase activity, and serum creatinine levels between\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n and wild\u2010type mice. Renal expression of nitro\u2010tyrosine, malondialdehyde, and urinary kidney injury marker\u20101 were increased in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice relative to wild\u2010type littermates.\n mRNA<\/jats:styled-content>\n expression of mitochondrial heat shock protein 60 was also elevated in kidneys from\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice, indicating the presence of oxidative stress. Tempol\u2010treated\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice presented higher serum nitrite\/nitrate levels than vehicle\u2010treated\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice, suggesting a role of the\n NO<\/jats:styled-content>\n system in the high blood pressure of this model. Tempol treatment normalized renal kidney injury marker\u20101 and malondialdehyde expression as well as blood pressure in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice, but had no effect in wild\u2010type mice. The renal\n Ucp2<\/jats:italic>\n mRNA<\/jats:styled-content>\n expression was increased in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice versus wild\u2010type and was also normalized by tempol. The renal\u2010selective silencing of\n Ucp2<\/jats:italic>\n led to normalization of blood pressure and serum nitrite\/nitrate ratio in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The deletion of\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n leads to oxidative stress\u2013induced hypertension associated with downregulation of\n NO<\/jats:styled-content>\n function, and overexpression of\n Ucp2<\/jats:italic>\n in the kidney increases blood pressure in\n \n DJ<\/jats:styled-content>\n \u20101\n <\/jats:italic>\n \n \u2212\/\u2212<\/jats:italic>\n <\/jats:sup>\n mice. To our knowledge, this is the first report providing evidence of the role of uncoupling protein 2 in blood pressure regulation.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.118.011856","type":"journal-article","created":{"date-parts":[[2019,4,18]],"date-time":"2019-04-18T09:04:28Z","timestamp":1555578268000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Uncoupling Protein 2 Increases Blood Pressure in DJ\u20101 Knockout Mice"],"prefix":"10.1161","volume":"8","author":[{"given":"Carmen","family":"De Miguel","sequence":"first","affiliation":[{"name":"Section\u00a0of Cardio\u2010Renal Physiology and Medicine Division of Nephrology Department of Medicine University of Alabama at Birmingham AL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William C.","family":"Hamrick","sequence":"additional","affiliation":[{"name":"Section\u00a0of Cardio\u2010Renal Physiology and Medicine Division of Nephrology Department of Medicine University of Alabama at Birmingham AL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randee","family":"Sedaka","sequence":"additional","affiliation":[{"name":"Section\u00a0of Cardio\u2010Renal Physiology and Medicine Division of Nephrology Department of Medicine University of Alabama at Birmingham AL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudha","family":"Jagarlamudi","sequence":"additional","affiliation":[{"name":"Division of Renal Diseases & Hypertension Department of Medicine The George Washington University School of Medicine and Health Sciences Washington DC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laureano D.","family":"Asico","sequence":"additional","affiliation":[{"name":"Division of Renal Diseases & Hypertension Department of Medicine The George Washington University School of Medicine and Health Sciences Washington DC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro A.","family":"Jose","sequence":"additional","affiliation":[{"name":"Division of Renal Diseases & Hypertension Department of Medicine The George Washington University School of Medicine and Health Sciences Washington DC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Cuevas","sequence":"additional","affiliation":[{"name":"Research Center for Genetic Medicine Children's National Health System Washington DC"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2016-310794"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5259"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05333.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M507124200"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6132"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.21831"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000254486.00883.3d"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms140917553"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/hypertensionaha.111.185744"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.114.04525"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2017.03.005"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.05.004"},{"key":"e_1_3_1_14_2","first-page":"2493","article-title":"Effect of uncoupling protein 2 on high\u2010glucose induced mitochondrial damage and apoptosis of cardiomyocytes","volume":"96","author":"Diao JY","year":"2016","unstructured":"Diao JY, Wei J, Yan R, Lin L, Li H. 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