{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:09:10Z","timestamp":1783523350280,"version":"3.55.0"},"reference-count":55,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,8,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Adenosine is a ubiquitous regulatory molecule known to modulate signaling in many cells and processes vital to vascular homeostasis. While studies of adenosine receptors have dominated research in the field, quantification of adenosine systemically and locally remains limited owing largely to technical restrictions. Given the potential clinical implications of adenosine biology, there is a need for adequately powered studies examining the role of plasma adenosine in vascular health. We sought to describe the analytical and biological factors that affect quantification of adenosine in humans in a large, real\u2010world cohort of patients undergoing evaluation for coronary artery disease.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Between November 2016 and April 2018, we assessed 1141 patients undergoing angiography for evaluation of coronary artery disease. High\u2010performance liquid chromatography was used for quantification of plasma adenosine concentration, yielding an analytical coefficient of variance (CV\n              <jats:sub>a<\/jats:sub>\n              ) of 3.2%, intra\u2010subject variance (CV\n              <jats:sub>i<\/jats:sub>\n              ) 35.8% and inter\u2010subject variance (CV\n              <jats:sub>g<\/jats:sub>\n              ) 56.7%. Traditional cardiovascular risk factors, medications, and clinical presentation had no significant impact on adenosine levels. Conversely, increasing age (\n              <jats:italic>P<\/jats:italic>\n              =0.027) and the presence of obstructive coronary artery disease (\n              <jats:italic>P<\/jats:italic>\n              =0.026) were associated with lower adenosine levels. Adjusted multivariable analysis supported only age being inversely associated with adenosine levels (\n              <jats:italic>P<\/jats:italic>\n              =0.039).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Plasma adenosine is not significantly impacted by traditional cardiovascular risk factors; however, advancing age and presence of obstructive coronary artery disease may be associated with lower adenosine levels. The degree of intra\u2010 and inter\u2010subject variance of adenosine has important implications for biomarker use as a prognosticator of cardiovascular outcomes and as an end point in clinical studies.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.012228","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T09:12:05Z","timestamp":1564996325000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["Evaluation of Plasma Adenosine as a Marker of Cardiovascular Risk: Analytical and Biological Considerations"],"prefix":"10.1161","volume":"8","author":[{"given":"Trevor","family":"Simard","sequence":"first","affiliation":[{"name":"CAPITAL Research Group Division of Cardiology University of Ottawa Heart Institute Ottawa Canada"},{"name":"Department of Cellular and Molecular Medicine University of Ottawa Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Jung","sequence":"additional","affiliation":[{"name":"CAPITAL Research Group Division of Cardiology University of Ottawa Heart Institute Ottawa Canada"},{"name":"Department of Cellular and Molecular Medicine University of Ottawa Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alisha","family":"Labinaz","sequence":"additional","affiliation":[{"name":"CAPITAL Research Group Division of Cardiology University of Ottawa Heart Institute Ottawa Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ali","family":"Faraz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine University of Ottawa Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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