{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:00:04Z","timestamp":1789696804289,"version":"4.0.1"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"14","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,7,16]]},"abstract":"\n Background<\/jats:title>\n \n Cardiac troponin T (\n cTnT<\/jats:styled-content>\n ) is seen in many other conditions besides myocardial infarction, and recent studies demonstrated distinct forms of\n cTnT<\/jats:styled-content>\n . At present, the in\u00a0vivo formation of these different\n cTnT<\/jats:styled-content>\n forms is incompletely understood. We therefore performed a study on the composition of\n cTnT<\/jats:styled-content>\n during the course of myocardial infarction, including coronary venous system sampling, close to its site of release.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Baseline samples were obtained from multiple coronary venous system locations, and a peripheral artery and vein in 71 non\u2013\n ST<\/jats:styled-content>\n \u2010segment\u2013elevation myocardial infarction patients. Additionally, peripheral blood was drawn at 6\u2010 and 12\u2010hours postcatheterization.\n cTnT<\/jats:styled-content>\n concentrations were measured using the high\u2010sensitivity\u2010\n cTnT<\/jats:styled-content>\n immunoassay. The\n cTnT<\/jats:styled-content>\n composition was determined via gel filtration chromatography and Western blotting in an early and late presenting patient. High\u2010sensitivity \u2010\n cTnT<\/jats:styled-content>\n concentrations were 28% higher in the coronary venous system than peripherally (n=71,\n P<\/jats:italic>\n <0.001). Coronary venous system samples demonstrated\n cT<\/jats:styled-content>\n n T\u2010I\u2010C complex, free intact\n cTnT<\/jats:styled-content>\n , and 29\n kD<\/jats:styled-content>\n a and 15 to 18\n kD<\/jats:styled-content>\n a\n cTnT<\/jats:styled-content>\n fragments, all in higher concentrations than in simultaneously obtained peripheral samples. While\n cT<\/jats:styled-content>\n n T\u2010I\u2010C complex proportionally decreased, and disappeared over time, 15 to 18\n kD<\/jats:styled-content>\n a\n cTnT<\/jats:styled-content>\n fragments increased. Moreover,\n cT<\/jats:styled-content>\n n T\u2010I\u2010C complex was more prominent in the early than in the late presenting patient.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This explorative study in non\u2013\n ST<\/jats:styled-content>\n \u2010segment\u2013elevation myocardial infarction shows that\n cTnT<\/jats:styled-content>\n is released from cardiomyocytes as a combination of\n cT<\/jats:styled-content>\n n T\u2010I\u2010C complex, free intact\n cTnT<\/jats:styled-content>\n , and multiple\n cTnT<\/jats:styled-content>\n fragments indicating intracellular\n cTnT<\/jats:styled-content>\n degradation. Over time, the\n cT<\/jats:styled-content>\n n T\u2010I\u2010C complex disappeared because of in\u00a0vivo degradation. These insights might serve as a stepping stone toward a high\u2010sensitivity\u2010\n cTnT<\/jats:styled-content>\n immunoassay more specific for myocardial infarction.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.119.012602","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T13:47:52Z","timestamp":1562248072000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["Multi\u2010Site Coronary Vein Sampling Study on Cardiac Troponin T Degradation in Non\u2013ST\u2010Segment\u2013Elevation Myocardial Infarction: Toward a More Specific Cardiac Troponin T Assay"],"prefix":"10.1161","volume":"8","author":[{"given":"Sander A. J.","family":"Damen","sequence":"first","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim H. M.","family":"Vroemen","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc A.","family":"Brouwer","sequence":"additional","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie T. P.","family":"Mezger","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Suryapranata","sequence":"additional","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"van Royen","sequence":"additional","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Otto","family":"Bekers","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J. R.","family":"Meex","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Will K. W. H.","family":"Wodzig","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freek W. A.","family":"Verheugt","sequence":"additional","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douwe","family":"de Boer","sequence":"additional","affiliation":[{"name":"Central Diagnostic Laboratory Maastricht University Medical Center Maastricht The Netherlands"},{"name":"CARIM School for Cardiovascular Diseases Maastricht University Maastricht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Etienne","family":"Cramer","sequence":"additional","affiliation":[{"name":"Department of Cardiology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alma M. 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