{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:07:14Z","timestamp":1790309234189,"version":"4.1.0"},"reference-count":60,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"17","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2019,9,3]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Cardiac arrest (CA) has been a leading cause of death for many decades. Despite years of research, we still do not understand how each organ responds to the reintroduction of blood flow after prolonged CA. Following changes in metabolites of individual organs after CA and resuscitation gives context to the efficiency and limitations of current resuscitation protocols.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">Adult male Sprague\u2013Dawley rats were arbitrarily assigned into 3 groups: control, 20\u00a0minutes of CA, or 20\u00a0minutes of CA followed by 30\u00a0minutes of cardiopulmonary bypass resuscitation. The rats were euthanized by decapitation to harvest brain, heart, kidney, and liver tissues. The obtained tissue samples were analyzed by ultra\u2010high\u2010performance liquid chromatography\u2013high\u2010accuracy mass spectrometry for comprehensive metabolomics evaluation. After resuscitation, the brain showed decreased glycolysis metabolites and fatty acids and increased amino acids compared with control. Similarly, the heart displayed alterations mostly in amino acids. The kidney showed decreased amino acid and fatty acid pools with severely increased tricarboxylic acid cycle metabolites following resuscitation, while the liver showed minimal alterations with slight changes in the lipid pool. Each tissue has a distinct pattern of metabolite changes after ischemia\/reperfusion. Furthermore, resuscitation worsens the metabolic dysregulation in the brain and kidney, while it normalizes metabolism in the heart.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Developing metabolic profiles using a global metabolome analysis identifies the variable nature of metabolites in individual organs after CA and reperfusion, establishing a stark contrast between the normalized heart and liver and the exacerbated brain and kidney, only after the reestablishment of blood circulation.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.012809","type":"journal-article","created":{"date-parts":[[2019,8,31]],"date-time":"2019-08-31T09:14:28Z","timestamp":1567242868000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":45,"title":["Tissue\u2010Specific Metabolic Profiles After Prolonged Cardiac Arrest Reveal Brain Metabolome Dysfunction Predominantly After Resuscitation"],"prefix":"10.1161","volume":"8","author":[{"given":"Jaewoo","family":"Choi","sequence":"first","affiliation":[{"name":"Linus Pauling Institute Oregon State University Corvallis OR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Shoaib","sequence":"additional","affiliation":[{"name":"Laboratory for Critical Care Physiology Feinstein Institute for Medical Research Manhasset NY"},{"name":"Department of Molecular Medicine Zucker School of Medicine at Hofstra\/Northwell Hempstead NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai","family":"Yin","sequence":"additional","affiliation":[{"name":"Laboratory for Critical Care Physiology Feinstein Institute for Medical Research Manhasset NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautam","family":"Nayyar","sequence":"additional","affiliation":[{"name":"Emory University Atlanta GA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Shinozaki","sequence":"additional","affiliation":[{"name":"Laboratory for Critical Care Physiology Feinstein Institute for Medical Research Manhasset NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan F.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Linus Pauling Institute Oregon State University Corvallis OR"},{"name":"Department of Pharmaceutical Sciences Oregon State University Corvallis OR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance B.","family":"Becker","sequence":"additional","affiliation":[{"name":"Laboratory for Critical Care Physiology Feinstein Institute for Medical Research Manhasset NY"},{"name":"Department of Molecular Medicine Zucker School of Medicine at Hofstra\/Northwell Hempstead NY"},{"name":"Department of Emergency Medicine North Shore University Hospital Manhasset NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory for Critical Care Physiology Feinstein Institute for Medical Research Manhasset NY"},{"name":"Department of Molecular Medicine Zucker School of Medicine at Hofstra\/Northwell Hempstead NY"},{"name":"Department of Emergency Medicine North Shore University Hospital Manhasset NY"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.110.988725"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ccx.0000127542.32890.fa"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezr315"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-0644(89)80257-4"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/314796"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000262"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep27187"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1310519"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394309-5.00006-7"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/mcs.a000588"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2016.09.018"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.3803\/EnM.2016.31.1.7"},{"key":"e_1_3_1_14_2","first-page":"294","article-title":"Metabolomics for the masses: the future of metabolomics in a personalized world","volume":"3","author":"Trivedi DK","year":"2017","unstructured":"Trivedi DK, Hollywood KA, Goodacre R. Metabolomics for the masses: the future of metabolomics in a personalized world. New Horiz Transl Med. 2017;3:294\u2013305.","journal-title":"New Horiz Transl Med"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-013-9505-9"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13909"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.112.000342"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-013-0588-8"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106647"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-016-0256-9"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0163021"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13793"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2006.03.024"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-015-1128-x"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010000260"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-015-2505-0"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2009.09.018"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.304495"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2006.01.018"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2010.08.032"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e318183f491"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3181feb339"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112012"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.020"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.129"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.02.055"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resuscitation.2013.01.019"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1995.129"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep36545"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2018.08.013"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7463407"},{"key":"e_1_3_1_42_2","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2015.1056156"},{"key":"e_1_3_1_43_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311002"},{"key":"e_1_3_1_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-018-0632-2"},{"key":"e_1_3_1_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26804-8"},{"key":"e_1_3_1_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2016.05.025"},{"key":"e_1_3_1_47_2","first-page":"4067","article-title":"Dynamic metabolites profile of cerebral ischemia\/reperfusion revealed by (1)h NMR\u2010based metabolomics contributes to potential biomarkers","volume":"7","author":"Wang Y","year":"2014","unstructured":"Wang Y, Wang YG, Ma TF, Li M, Gu SL. Dynamic metabolites profile of cerebral ischemia\/reperfusion revealed by (1)h NMR\u2010based metabolomics contributes to potential biomarkers. Int J Clin Exp Pathol. 2014;7:4067\u20134075.","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_3_1_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.09.008"},{"key":"e_1_3_1_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep22415"},{"key":"e_1_3_1_50_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/792391"},{"key":"e_1_3_1_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165884"},{"key":"e_1_3_1_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.10.020"},{"key":"e_1_3_1_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16857-6"},{"key":"e_1_3_1_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.04.104"},{"key":"e_1_3_1_55_2","doi-asserted-by":"publisher","DOI":"10.1258\/la.2007.006035"},{"key":"e_1_3_1_56_2","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2014.0530"},{"key":"e_1_3_1_57_2","doi-asserted-by":"publisher","DOI":"10.1079\/PHN2005795"},{"key":"e_1_3_1_58_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31828a39e9"},{"key":"e_1_3_1_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2008.03.015"},{"key":"e_1_3_1_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcad.2012.06.004"},{"key":"e_1_3_1_61_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc13907"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.119.012809","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:36:30Z","timestamp":1715600190000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.119.012809"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,3]]},"references-count":60,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2019,9,3]]}},"alternative-id":["10.1161\/JAHA.119.012809"],"URL":"https:\/\/doi.org\/10.1161\/jaha.119.012809","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,3]]},"assertion":[{"value":"2019-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-31","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e012809"}}