{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:02:22Z","timestamp":1793149342832,"version":"4.5.0"},"reference-count":52,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,1,21]]},"abstract":"\n Background<\/jats:title>\n \n Poor engraftment of intramyocardial stem cells limits their therapeutic efficiency against myocardial infarction (\n MI<\/jats:styled-content>\n )\u2010induced cardiac injury. Transglutaminase cross\u2010linked Gelatin (Col\u2010Tgel) is a tailorable collagen\u2010based hydrogel that is becoming an excellent biomaterial scaffold for cellular delivery in\u00a0vivo. Here, we tested the hypothesis that Col\u2010Tgel increases retention of intramyocardially\u2010injected stem cells, and thereby reduces post\u2010\n MI<\/jats:styled-content>\n cardiac injury.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Adipose\u2010derived mesenchymal stem cells (\n ADSC<\/jats:styled-content>\n s) were co\u2010cultured with Col\u2010Tgel in a 3\u2010dimensional system in\u00a0vitro, and Col\u2010Tgel encapsulated\n ADSC<\/jats:styled-content>\n s were observed using scanning electron microscopy and confocal microscopy. Vitality, proliferation, and migration of co\u2010cultured\n ADSC<\/jats:styled-content>\n s were evaluated. In addition, mice were subjected to\n MI<\/jats:styled-content>\n and were intramyocardially injected with\n ADSC<\/jats:styled-content>\n s, Col\u2010Tgel, or a combination thereof.\n ADSC<\/jats:styled-content>\n s engraftment, survival, cardiac function, and fibrosis were assessed. In vitro\n MTT<\/jats:styled-content>\n and Cell Counting Kit\u20108 assays demonstrated that\n ADSC<\/jats:styled-content>\n s survive and proliferate up to 4\u00a0weeks in the Col\u2010Tgel. In addition,\n MTT<\/jats:styled-content>\n and transwell assays showed that\n ADSC<\/jats:styled-content>\n s migrate outside the edge of the Col\u2010Tgel sphere. Furthermore, when compared with\n ADSC<\/jats:styled-content>\n s alone, Col\u2010Tgel\u2010encapsulated\n ADSC<\/jats:styled-content>\n s significantly enhanced the long\u2010term retention and cardioprotective effect of\n ADSC<\/jats:styled-content>\n s against\n MI<\/jats:styled-content>\n \u2010induced cardiac injury.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n In the current study, we successfully established a 3\u2010dimensional co\u2010culture system using\n ADSC<\/jats:styled-content>\n s and Col\u2010Tgel. The Col\u2010Tgel creates a suitable microenvironment for long\u2010term retention of\n ADSC<\/jats:styled-content>\n s in an ischemic area, and thereby enhances their cardioprotective effects. Taken together, this study may provide an alternative biomaterial for stem cell\u2010based therapy to treat ischemic heart diseases.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.119.013784","type":"journal-article","created":{"date-parts":[[2020,1,18]],"date-time":"2020-01-18T05:00:26Z","timestamp":1579323626000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["Tailorable Hydrogel Improves Retention and Cardioprotection of Intramyocardial Transplanted Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice"],"prefix":"10.1161","volume":"9","author":[{"given":"Youhu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cardiology Xijing Hospital Fourth Military Medical University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congye","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology Xijing Hospital 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