{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T14:09:33Z","timestamp":1776262173083,"version":"3.50.1"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"16","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,8,18]]},"abstract":"\n Background<\/jats:title>\n \n Basal release of nitric oxide (\n NO<\/jats:styled-content>\n ) from the vascular endothelium regulates the tone of muscular arteries and resistance vasculature. Effects of\n NO<\/jats:styled-content>\n on muscular arteries could be particularly important during exercise when shear stress may stimulate increased\n NO<\/jats:styled-content>\n synthesis.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n We investigated acute effects of\n NO<\/jats:styled-content>\n synthase inhibition on exercise hemodynamics using\n \n N\n G<\/jats:sup>\n <\/jats:styled-content>\n \u2010monomethyl\u2010\n l<\/jats:sc>\n \u2010arginine (\n l<\/jats:sc>\n \u2010\n NMMA<\/jats:styled-content>\n ), a nonselective\n NO<\/jats:styled-content>\n synthase \u2010inhibitor. Healthy volunteers (n=10, 5 female, 19\u201333\u00a0years) participated in a 2\u2010phase randomized crossover study, receiving\n l<\/jats:sc>\n \u2010\n NMMA<\/jats:styled-content>\n (6\u00a0mg\/kg, iv over 5\u00a0minutes) or placebo before bicycle exercise (25\u2013150\u00a0W for 12\u00a0minutes). Blood pressure, cardiac output (measured by dilution of soluble and inert tracers) and femoral artery diameter were measured before, during, and after exercise. At rest,\n l<\/jats:sc>\n \u2010\n NMMA<\/jats:styled-content>\n reduced heart rate (by 16.2\u00b14.3\u00a0bpm relative to placebo,\n P<\/jats:italic>\n <0.01), increased peripheral vascular resistance (by 7.0\u00b11.4\u00a0mmHg per L\/min,\n P<\/jats:italic>\n <0.001), mean arterial blood pressure (by 8.9\u00b13.5\u00a0mmHg,\n P<\/jats:italic>\n <0.05), and blunted an increase in femoral artery diameter that occurred immediately before exercise (change in diameter: 0.14\u00b10.04 versus 0.32\u00b10.06\u00a0mm after\n l<\/jats:sc>\n \u2010\n NMMA<\/jats:styled-content>\n and placebo,\n P<\/jats:italic>\n <0.01). During\/after exercise\n l<\/jats:sc>\n \u2010\n NMMA<\/jats:styled-content>\n had no significant effect on peripheral resistance, cardiac output, or on femoral artery diameter.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n These results suggest that\n NO<\/jats:styled-content>\n plays little role in modulating muscular artery function during exercise but that it may mediate changes in muscular artery tone immediately before exercise.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.119.013849","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T08:58:36Z","timestamp":1597222716000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of Inhibition of Nitric Oxide Synthase on Muscular Arteries During Exercise: Nitric Oxide Does Not Contribute to Vasodilation During Exercise or in Recovery"],"prefix":"10.1161","volume":"9","author":[{"given":"Kevin","family":"O'Gallagher","sequence":"first","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Husain","family":"Shabeeh","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahzad","family":"Munir","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Roomi","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benyu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antoine","family":"Guilcher","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sally","family":"Brett","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"Chowienczyk","sequence":"additional","affiliation":[{"name":"Cardiovascular Division Department of Clinical Pharmacology King\u2019s College London School of Medicine St Thomas\u2019 Hospital London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/hy1101.095329"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/hc0202.101970"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.91.5.1314"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.2.145"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.096016"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01171.2007"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199312000-00009"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.36.6.952"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.76.6.2832"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0895-7061(01)02252-X"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199816040-00004"},{"key":"e_1_3_1_13_2","first-page":"F364","article-title":"Physiological role of nitric oxide in regulation of renal function in humans","author":"Haynes WG","year":"1997","unstructured":"Haynes WG, Hand MF, Dockrell ME, Eadington DW, Lee MR, Hussein Z, Benjamin N, Webb DJ. Physiological role of nitric oxide in regulation of renal function in humans. Am J Physiol. 1997;F364\u2013F371.","journal-title":"Am J Physiol"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.015107"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00179.2004"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carrev.2018.10.010"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00084a017"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47072-X"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.822205"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.119.013849","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:56:41Z","timestamp":1715601401000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.119.013849"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,18]]},"references-count":18,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2020,8,18]]}},"alternative-id":["10.1161\/JAHA.119.013849"],"URL":"https:\/\/doi.org\/10.1161\/jaha.119.013849","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,18]]},"assertion":[{"value":"2020-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e013849"}}