{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T02:58:45Z","timestamp":1768445925506,"version":"3.49.0"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,2,4]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              The clinical aspects of sickle cell anemia (\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              ) are heterogeneous, and different patients may present significantly different clinical evolutions. Almost all organs can be affected, particularly the central nervous system. Transient ischemic events, infarcts, and cerebral hemorrhage can be observed and affect \u224825% of the patients with\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              . Differences in the expression of molecules produced by endothelial cells may be associated with the clinical heterogeneity of patients affected by vascular diseases. In this study, we investigated the differential expression of genes involved in endothelial cell biology in\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              patients with and without stroke.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Endothelial progenitor cells from 4\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              patients with stroke and 6\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              patients without stroke were evaluated through the polymerase chain reaction array technique. The analysis of gene expression profiling identified 29 differentially expressed genes. Eleven of these genes were upregulated, and most were associated with angiogenesis (55%), inflammatory response (18%), and coagulation (18%) pathways. Downregulated expression was observed in 18 genes, with the majority associated with angiogenesis (28%), apoptosis (28%), and cell adhesion (22%) pathways. Remarkable overexpression of the\n              <jats:italic>\n                <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                1\n              <\/jats:italic>\n              (matrix metalloproteinase 1) gene in the endothelial progenitor cells of all\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              patients with stroke (fold change: 204.64;\n              <jats:italic>P<\/jats:italic>\n              =0.0004) was observed.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Our results strongly suggest that angiogenesis is an important process in sickle cell stroke, and differences in the gene expression profile of endothelial cell biology, especially\n              <jats:italic>\n                <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\n                1\n              <\/jats:italic>\n              , may be related to stroke in\n              <jats:styled-content style=\"fixed-case\">SCA<\/jats:styled-content>\n              patients.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.014143","type":"journal-article","created":{"date-parts":[[2020,2,3]],"date-time":"2020-02-03T11:12:30Z","timestamp":1580728350000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["Angiogenesis\u2010Related Genes in Endothelial Progenitor Cells May Be Involved in Sickle Cell Stroke"],"prefix":"10.1161","volume":"9","author":[{"given":"Mirta T.","family":"Ito","sequence":"first","affiliation":[{"name":"Center for Molecular Biology and Genetic Engineering University of Campinas\u2010UNICAMP Campinas S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sueli 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