{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:57:08Z","timestamp":1792670228594,"version":"4.5.0"},"reference-count":35,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,3,3]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Neutrophils play a major role in inflammation after myocardial ischemia\u2010reperfusion (I\/R) injury. The effects of mesenchymal stem cells (MSCs) on neutrophils in I\/R are complex and not fully understood. This study was designed to investigate the effects and mechanism of MSCs on alleviating myocardial I\/R injury in rats.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">MSCs induced M2 macrophages polarization in\u00a0vitro and enhanced macrophage efferocytosis of apoptotic neutrophils, measured by fluorescence\u2010activated cell sorting analysis and immunofluorescence staining. Rats myocardial I\/R were induced by transient ligation of left anterior descending coronary. Adipose\u2010derived MSCs or vehicle were infused at initiation (immediate after reperfusion) or peak of inflammation (24\u00a0hours after I\/R). Hematoxylin and eosin, 2,3,5\u2010triphenyltetrazolium chloride\/Evans Blue staining and immunofluorescence staining were applied within 72\u00a0hours after cell infusion. Cardiac function was assessed by echocardiography and left cardiac catheterization analysis at 28\u00a0days post\u2010operation. MSCs infused immediately and 24\u00a0hours later both markedly ameliorated myocardial I\/R injury, and immediate infusion had more significant outcome. These improvements were associated with neutrophils infiltration, measured by fluorescence\u2010activated cell sorting analysis and immunofluorescence staining. When infused immediately, MSCs did not significantly change neutrophil number at 24\u00a0hours but CD11b expression was significantly higher. When infused at 24\u00a0hours, MSCs markedly decreased neutrophil number by enhanced M2 macrophage infiltration and macrophage efferocytosis of neutrophils within 72\u00a0hours.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Efferocytosis is pivotal to relieve neutrophil\u2010mediated I\/R injury and initial the immune response for healing. MSCs infusion improves cardiac function in rats after myocardial I\/R via the possible mechanism of enhancing M2 macrophages\u2010induced efferocytosis of apoptotic neutrophils.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.014397","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T10:00:23Z","timestamp":1582279223000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":40,"title":["Mesenchymal Stem Cells Promote the Resolution of Cardiac Inflammation After Ischemia Reperfusion Via Enhancing Efferocytosis of Neutrophils"],"prefix":"10.1161","volume":"9","author":[{"given":"Zeyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiology Chinese PLA General Hospital Beijing China"},{"name":"Beijing Key Laboratory of Chronic Heart Failure Precision Medicine Chinese PLA General Hospital Beijing 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