{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:56:45Z","timestamp":1792652205334,"version":"4.5.0"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,6,2]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Current mammalian models for heart regeneration research are limited to neonatal apex amputation and myocardial infarction, both of which are controversial.\n              <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n              seq has demonstrated a very limited set of differentially expressed genes between sham and operated hearts in myocardial infarction models. Here, we investigated in rats whether pressure overload in the right ventricle, a common phenomenon in children with congenital heart disease, could be used as a better animal model for heart regeneration studies when considering cardiomyocyte proliferation as the most important index.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              In the rat model, pressure overload was induced by pulmonary artery banding on postnatal day 1 and confirmed by echocardiography and hemodynamic measurements at postnatal day 7.\n              <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n              sequencing analyses of purified right ventricular cardiomyocytes at postnatal day 7 from pulmonary artery banding and sham\u2010operated rats revealed that there were 5469 differentially expressed genes between these 2 groups. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis showed that these genes mainly mediated mitosis and cell division. Cell proliferation assays indicated a continuous overproliferation of cardiomyocytes in the right ventricle after pulmonary artery banding, in particular for the first 3 postnatal days. We also validated the model using samples from overloaded right ventricles of human patients. There was an approximately 2\u2010fold increase of Ki67\/\n              <jats:styled-content style=\"fixed-case\">pHH<\/jats:styled-content>\n              3\/aurora B\u2010positive cardiomyocytes in human\u2010overloaded right ventricles compared with nonoverloaded right ventricles. Other features of this animal model included cardiomyocyte hypotrophy with no fibrosis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Pressure overload profoundly promotes cardiomyocyte proliferation in the neonatal stage in both rats and human beings. This activates a regeneration\u2010specific gene program and may offer an alternative animal model for heart regeneration research.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.015574","type":"journal-article","created":{"date-parts":[[2020,5,30]],"date-time":"2020-05-30T09:00:18Z","timestamp":1590829218000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":31,"title":["Pressure Overload Greatly Promotes Neonatal Right Ventricular Cardiomyocyte Proliferation: A New Model for the Study of Heart Regeneration"],"prefix":"10.1161","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-2810","authenticated-orcid":false,"given":"Lincai","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Institute of Pediatric Translational Medicine Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Shanghai Institute for Pediatric Congenital Heart Disease Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoubao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Department of Plastic and Reconstructive Surgery Shanghai Ninth People\u2019s Hospital Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingying","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Institute of Pediatric Translational Medicine Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Shanghai Institute for Pediatric Congenital Heart Disease Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhui","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiwen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haibo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"},{"name":"Shanghai Institute for Pediatric Congenital Heart Disease Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoming","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifa","family":"Hong","sequence":"additional","affiliation":[{"name":"Institute of Pediatric Translational Medicine Shanghai Children\u2019s Medical Center Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.040682"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2017.09.014"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101128"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22979"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1905824116"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.039276"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2015.11.031"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.06.003"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.028252"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.12.011"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100526"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2018.07.007"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.128336"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccl.2015.07.002"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000002238"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12833"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clp.2017.08.001"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(94)70373-6"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00163.2011"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.927988"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2017.06.016"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep23188"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa5171"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000429"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1200708"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aao5553"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10135-x"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.315229"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.119.015574","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:34:26Z","timestamp":1715600066000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.119.015574"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,2]]},"references-count":28,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,6,2]]}},"alternative-id":["10.1161\/JAHA.119.015574"],"URL":"https:\/\/doi.org\/10.1161\/jaha.119.015574","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,2]]},"assertion":[{"value":"2019-12-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e015574"}}