{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:11:26Z","timestamp":1790172686962,"version":"4.1.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,4,21]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Human relaxin\u20102 is a peptide hormone capable of pleiotropic effects in several organ systems. Its recombinant formulation (serelaxin) has been demonstrated to reduce infarct size and prevent excessive scar formation in animal models of cardiac ischemia\u2010reperfusion injury. B7\u201033, a synthetically designed peptide analogous to B\u2010chain of relaxin\u20102, invokes signaling at relaxin family peptide receptor 1 (cognate receptor for relaxin\u20102) by preferentially phosphorylating the mitogen\u2010activated protein kinase extracellular signal\u2010regulated kinase 1\/2. We sought to investigate the effects of B7\u201033 treatment post ischemia\u2010reperfusion injury in mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Adult male\n              <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n              1 mice were subjected to ischemia\u2010reperfusion via ligation of left anterior descending artery for 30\u00a0minutes, followed by 24\u00a0hours or 7\u00a0days of reperfusion. Echocardiography was performed to assess cardiac function, and cardiac tissue was stained to determine infarct size at 24\u00a0hours. B7\u201033 significantly reduced infarct size (21.99% versus 45.32%;\n              <jats:italic>P<\/jats:italic>\n              =0.02) and preserved fractional shortening (29% versus 23%;\n              <jats:italic>P<\/jats:italic>\n              =0.02) compared with vehicle. The difference in fractional shortening further increased at 7\u00a0days post myocardial infarction (29% versus 20% for B7\u201033 and vehicle groups, respectively). In vitro\n              <jats:italic>,<\/jats:italic>\n              primary cardiomyocytes were isolated from adult hearts and subjected to simulated ischemia\u2010reperfusion injury (simulated ischemia reoxygenation). B7\u201033 (50 and 100\u00a0nmol\/L) improved cell survival and reduced the expression of\n              <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>\n              78 (glucose regulated protein), an endoplasmic reticulum stress marker. Subsequently, B7\u201033 (100\u00a0nmol\/L) reduced tunicamycin (2.5\u00a0\u03bcg\/mL) induced upregulation of\n              <jats:styled-content style=\"fixed-case\">GRP<\/jats:styled-content>\n              78 in an extracellular signal\u2010regulated kinase 1\/2\u2013dependent manner.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">B7\u201033 confers acute cardioprotection and limits myocardial infarction\u2013related adverse remodeling in mice by attenuating cardiomyocyte death and endoplasmic reticulum stress as well as preserving cardiac function.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.015748","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T09:00:34Z","timestamp":1587027634000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["B7\u201033, a Functionally Selective Relaxin Receptor 1 Agonist, Attenuates Myocardial Infarction\u2013Related Adverse Cardiac Remodeling in Mice"],"prefix":"10.1161","volume":"9","author":[{"given":"Teja","family":"Devarakonda","sequence":"first","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo G.","family":"Mauro","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geronimo","family":"Guzman","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahak","family":"Hovsepian","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chad","family":"Cain","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anindita","family":"Das","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Praveen","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health University of Melbourne Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Akhter","family":"Hossain","sequence":"additional","affiliation":[{"name":"Florey Institute of Neuroscience and Mental Health University of Melbourne Parkville Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8947-9315","authenticated-orcid":false,"given":"Fadi N.","family":"Salloum","sequence":"additional","affiliation":[{"name":"Division of Cardiology Pauley Heart Center Virginia Commonwealth University Richmond VA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","first-page":"1","article-title":"Reperfusion therapy with recombinant human relaxin\u20102 (serelaxin) attenuates myocardial infarct size and NLRP3 inflammasome following ischemia\/reperfusion injury via eNOS\u2010dependent mechanism","volume":"2","author":"Valle Raleigh J","year":"2017","unstructured":"Valle Raleigh J, Mauro AG, Devarakonda T, Marchetti C, He J, Kim E, Filippone S, Das A, Toldo S, Abbate A, et\u00a0al. 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