{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:20:47Z","timestamp":1785892847292,"version":"3.56.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"16","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,8,18]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Extracellular matrix, especially laminin\u2010221, may play crucial roles in viability and survival of human\u2010induced pluripotent stem cell\u2010derived cardiomyocytes (hiPS\u2010CMs) after in vivo transplant. Then, we hypothesized laminin\u2010221 may have an adjuvant effect on therapeutic efficacy by enhancing cell viability and survival after transplantation of 3\u2010dimensional engineered cardiac tissue (ECT) to a rat model of myocardial infarction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">In vitro study indicates the impacts of laminin\u2010221 on hiPS\u2010CMs were analyzed on the basis of mechanical function, mitochondrial function, and tolerance to hypoxia. We constructed 3\u2010dimensional ECT containing hiPS\u2010CMs and fibrin gel conjugated with laminin\u2010221. Heart function and in vivo behavior were assessed after engraftment of 3\u2010dimensional ECT (laminin\u2010conjugated ECT, n=10; ECT, n=10; control, n=10) in a rat model of myocardial infarction. In vitro assessment indicated that laminin\u2010221 improves systolic velocity, diastolic velocity, and maximum capacity of oxidative metabolism of hiPS\u2010CMs. Cell viability and lactate dehydrogenase production revealed that laminin\u2010221 improved tolerance to hypoxia. Furthermore, analysis of mRNA expression revealed that antiapoptotic genes were upregulated in the laminin group under hypoxic conditions. Left ventricular ejection fraction of the laminin\u2010conjugated ECT group was significantly better than that of other groups 4 weeks after transplantation. Laminin\u2010conjugated ECT transplantation was associated with significant improvements in expression levels of rat vascular endothelial growth factor. In early assessments, cell survival was also improved in laminin\u2010conjugated ECTs compared with ECT transplantation without laminin\u2010221.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">In vitro laminin\u2010221 enhanced mechanical and metabolic function of hiPS\u2010CMs and improved the therapeutic impact of 3\u2010dimensional ECT in a rat ischemic cardiomyopathy model. These findings suggest that adjuvant laminin\u2010221 may provide a clinical benefit to hiPS\u2010CM constructs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.119.015841","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T11:52:57Z","timestamp":1597233177000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":19,"title":["Laminin\u2010221 Enhances Therapeutic Effects of Human\u2010Induced Pluripotent Stem Cell\u2013Derived 3\u2010Dimensional Engineered Cardiac Tissue Transplantation in a Rat Ischemic Cardiomyopathy Model"],"prefix":"10.1161","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3514-4413","authenticated-orcid":false,"given":"Takaaki","family":"Samura","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery Osaka University Graduate School of Medicine Osaka 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