{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:55:00Z","timestamp":1787018100970,"version":"build-2736575974"},"reference-count":41,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"22","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,11,17]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Failure rates after revascularization surgery remain high, both in vein grafts (VG) and arterial interventions. One promising approach to improve outcomes is endogenous upregulation of the gaseous transmitter\u2010molecule hydrogen sulfide, via short\u2010term dietary restriction. However, strict patient compliance stands as a potential translational barrier in the vascular surgery patient population. Here we present a new therapeutic approach, via a locally applicable gel containing the hydrogen sulfide releasing prodrug (GYY), to both mitigate graft failure and improve arterial remodeling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">All experiments were performed on C57BL\/6 (male, 12 weeks old) mice. VG surgery was performed by grafting a donor\u2010mouse cava vein into the right common carotid artery of a recipient via an end\u2010to\u2010end anastomosis. In separate experiments arterial intimal hyperplasia was assayed via a right common carotid artery focal stenosis model. All mice were harvested at postoperative day 28 and artery\/graft was processed for histology. Efficacy of hydrogen sulfide was first tested via GYY supplementation of drinking water either 1 week before VG surgery (pre\u2010GYY) or starting immediately postoperatively (post\u2010GYY). Pre\u2010GYY mice had a 36.5% decrease in intimal\/media+adventitia area ratio compared with controls. GYY in a 40% Pluronic gel (or vehicle) locally applied to the graft\/artery had decreased intimal\/media area ratios (right common carotid artery) and improved vessel diameters. GYY\u2010geltreated VG had larger diameters at both postoperative days 14 and 28, and a 56.7% reduction in intimal\/media+adventitia area ratios. Intimal vascular smooth muscle cell migration was decreased 30.6% after GYY gel treatment, which was reproduced in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Local gel\u2010based treatment with the hydrogen sulfide\u2010donor GYY stands as a translatable therapy to improve VG durability and arterial remodeling after injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.120.016391","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T05:28:03Z","timestamp":1604467683000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":21,"title":["Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft Disease"],"prefix":"10.1161","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7113-2068","authenticated-orcid":false,"given":"Peter","family":"Kip","sequence":"first","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"},{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"},{"name":"Einthoven Laboratory for Experimental Vascular Medicine and Department of Surgery Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaspar M.","family":"Trocha","sequence":"additional","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"},{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"MacArthur","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrika A. B.","family":"Peters","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine and Department of Surgery Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte G.","family":"Mann","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijs J.","family":"Sluiter","sequence":"additional","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"},{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"},{"name":"Einthoven Laboratory for Experimental Vascular Medicine and Department of Surgery Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0674-9066","authenticated-orcid":false,"given":"Jonathan","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"},{"name":"School of Medicine University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzannah","family":"Patterson","sequence":"additional","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6853-5760","authenticated-orcid":false,"given":"Paul H. A.","family":"Quax","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine and Department of Surgery Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margreet R.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Einthoven Laboratory for Experimental Vascular Medicine and Department of Surgery Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James R.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Molecular Metabolism Harvard T.H. Chan School of Public Health Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9906-6218","authenticated-orcid":false,"given":"C.","family":"Keith Ozaki","sequence":"additional","affiliation":[{"name":"Department of Surgery and the Heart and Vascular Center Brigham &amp; Women\u2019s Hospital Harvard Medical School Boston MA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2018.01.066"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1067\/mva.2002.86"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2005.12.058"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2016.76"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2018.00003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2012.11.001"},{"key":"e_1_3_2_8_2","first-page":"118ra11","article-title":"Surgical stress resistance induced by single amino acid deprivation requires Gcn2 in mice","author":"Peng W","year":"2012","unstructured":"Peng W, Robertson L, Gallinetti J, Mejia P, Vose S, Charlip A, Chu T, Mitchell JR. 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