{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:06:18Z","timestamp":1792649178340,"version":"4.5.0"},"reference-count":47,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"19","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2020,10,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Hydrogen sulfide (H\n              <jats:sub>2<\/jats:sub>\n              S) is an important endogenous physiological signaling molecule and exerts protective properties in the cardiovascular system. Cystathionine \u03b3\u2010lyase (CSE), 1 of 3 H\n              <jats:sub>2<\/jats:sub>\n              S producing enzyme, is predominantly localized in the vascular endothelium. However, the regulation of CSE in vascular endothelium remains incompletely understood.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              We generated inducible endothelial cell\u2010specific CSE overexpressed transgenic mice (EC\u2010CSE Tg) and endothelial cell\u2010specific CSE knockout mice (EC\u2010CSE KO), and investigated vascular function in isolated thoracic aorta, treadmill exercise capacity, and myocardial injury following ischemia\u2010reperfusion in these mice. Overexpression of CSE in endothelial cells resulted in increased circulating and myocardial H\n              <jats:sub>2<\/jats:sub>\n              S and NO, augmented endothelial\u2010dependent vasorelaxation response in thoracic aorta, improved exercise capacity, and reduced myocardial\u2010reperfusion injury. In contrast, genetic deletion of CSE in endothelial cells led to decreased circulating H\n              <jats:sub>2<\/jats:sub>\n              S and cardiac NO production, impaired endothelial dependent vasorelaxation response and reduced exercise capacity. However, myocardial\u2010reperfusion injury was not affected by genetic deletion of endothelial cell CSE.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              CSE\u2010derived H\n              <jats:sub>2<\/jats:sub>\n              S production in endothelial cells is critical in maintaining endothelial function, exercise capacity, and protecting against myocardial ischemia\/reperfusion injury. Our data suggest that the endothelial NO synthase\u2014NO pathway is likely involved in the beneficial effects of overexpression of CSE in the endothelium.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.120.017544","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T09:30:41Z","timestamp":1601371841000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":43,"title":["Endothelial Cell Cystathionine \u03b3\u2010Lyase Expression Level Modulates Exercise Capacity, Vascular Function, and Myocardial Ischemia Reperfusion Injury"],"prefix":"10.1161","volume":"9","author":[{"given":"Huijing","family":"Xia","sequence":"first","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Li","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8706-9825","authenticated-orcid":false,"suffix":"III","given":"Thomas E.","family":"Sharp","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Polhemus","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Carnal","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4816-4915","authenticated-orcid":false,"given":"Karl H.","family":"Moles","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Xiong","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology, and Pharmacology College of Veterinary Medicine Auburn University Auburn AL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3925-2224","authenticated-orcid":false,"given":"John","family":"Elrod","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine Lewis Katz School of Medicine Temple University Philadelphia PA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"Pfeilschifter","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology Goethe University Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl\u2010Friedrich","family":"Beck","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology and Toxicology Goethe University Frankfurt am Main Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2293-7278","authenticated-orcid":false,"given":"David J.","family":"Lefer","sequence":"additional","affiliation":[{"name":"Cardiovascular Center of Excellence Louisiana State University Health Sciences Center New Orleans LA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00329.2016"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.300505"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2008.2282"},{"key":"e_1_3_1_5_2","first-page":"805","article-title":"Hydrogen sulfide and the pathogenesis of atherosclerosis","author":"Sarathi M","year":"2014","unstructured":"Sarathi M, Ashley U, Lingyun W, Rui W. 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