{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:35:19Z","timestamp":1791909319443,"version":"4.3.4"},"reference-count":69,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2021,3,16]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Brain\u2010derived neurotrophic factor (BDNF) is expressed in neuronal and nonneuronal cells and may affect vascular functions via its receptor, tropomyosin\u2010related kinase B (TrkB). In this study, we determined the expression of BDNF in different perivascular adipose tissue (PVAT) depots of patients with established coronary atherosclerosis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Serum, vascular tissue, and PVAT surrounding the proximal aorta (C\u2010PVAT) or internal mammary artery (IMA\u2010PVAT) was obtained from 24 patients (79% men; mean age, 71.7\u00b19.7\u00a0years; median body mass index, 27.4\u00b14.8\u00a0kg\/m\n              <jats:sup>2<\/jats:sup>\n              ) with coronary atherosclerosis undergoing elective coronary artery bypass surgery. BDNF protein levels were significantly higher in C\u2010PVAT compared with IMA\u2010PVAT, independent of obesity, metabolic syndrome, or systemic biomarkers of inflammation. mRNA transcripts of TrkB, the BDNF receptor, were significantly reduced in aorta compared with IMA. Vessel wall TrkB immunosignals colocalized with cells expressing smooth muscle cell markers, and confocal microscopy and flow cytometry confirmed BDNF receptor expression in human aortic smooth muscle cells. Significantly elevated levels of protein tyrosine phosphatase 1B, a negative regulator of TrkB signaling in the brain, were also observed in C\u2010PVAT. In vitro, inhibition of protein tyrosine phosphatase 1B blunted the effects of BDNF on smooth muscle cell proliferation, migration, differentiation, and collagen production, possibly by upregulation of low\u2010affinity p75 neurotrophin receptors. Expression of nerve growth factor or its receptor tropomyosin\u2010related kinase A did not differ between C\u2010PVAT and IMA\u2010PVAT.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Elevated expression of BDNF in parallel with local upregulation of negative regulators of neurotrophin signaling in perivascular fat and lower TrkB expression suggest that vascular BDNF signaling is reduced or lost in patients with coronary atherosclerosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.120.018322","type":"journal-article","created":{"date-parts":[[2021,3,5]],"date-time":"2021-03-05T11:16:23Z","timestamp":1614942983000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":31,"title":["Brain\u2010Derived Neurotrophic Factor Expression and Signaling in Different Perivascular Adipose Tissue Depots of Patients With Coronary Artery Disease"],"prefix":"10.1161","volume":"10","author":[{"given":"Sarah","family":"Zierold","sequence":"first","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Buschmann","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic and Vascular Surgery University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sogol","family":"Gachkar","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena L.","family":"Bochenek","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"},{"name":"Center for Thrombosis and Hemostasis University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Velmeden","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas","family":"Hobohm","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"},{"name":"Center for Thrombosis and Hemostasis University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian\u2010Friedrich","family":"Vahl","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic and Vascular Surgery University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0846-4718","authenticated-orcid":false,"given":"Katrin","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Department of Cardiology Cardiology I University Medical Center Mainz Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7624777"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7624776"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1042\/CS20160044"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.09.065"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.6.1290"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0550"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn1336"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1815744116"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-1166"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2018.06.007"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00475.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00076.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/4537274"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-06-0716"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000005260"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010099"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_1566_17"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.10.020"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2004.10.041"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12872-018-0762-z"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.114.001544"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.104.476903"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00455"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.587980"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.21.4531"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8418"},{"key":"e_1_3_2_28_2","first-page":"309","article-title":"Neurotrophin and neurotrophin receptors in vascular smooth muscle cells. Regulation of expression in response to injury","volume":"147","author":"Donovan MJ","year":"1995","unstructured":"Donovan MJ, Miranda RC, Kraemer R, McCaffrey TA, Tessarollo L, Mahadeo D, Sharif S, Kaplan DR, Tsoulfas P, Parada L. Neurotrophin and neurotrophin receptors in vascular smooth muscle cells. Regulation of expression in response to injury. Am J Pathol. 1995;147:309\u2013324.","journal-title":"Am J Pathol"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.08.028"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/ijo.2017.121"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.113.301393"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00392-016-0996-7"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1159\/000350542"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000111245.75752.C6"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309798"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-8703(83)90478-7"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90628-C"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19082281"},{"key":"e_1_3_2_39_2","first-page":"3246","article-title":"Obesity itself does not influence BDNF serum levels in adults","volume":"18","author":"Gajewska E","year":"2014","unstructured":"Gajewska E, Sobieska M, Lojko D, Wieczorowska\u2010Tobis K, Suwalska A. Obesity itself does not influence BDNF serum levels in adults. Eur Rev Med Pharmacol Sci. 2014;18:3246\u20133250.","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jada.2006.03.015"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000025241.01418.4D"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2015.04.003"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(03)46018-4"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00077.2019"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01302-8"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2006.09.001"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.02.014"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.720029"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8071000"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.01.006"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22811"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129489"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4969523"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1385\/MN:27:3:277"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2002.01891.x"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087406"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108989200"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64640-8"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22701"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200701040"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81294-5"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1295-1311"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.603621"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05686.x"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1435"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI80323"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2010.01.005"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1996.tb01290.x"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2019.111504"},{"key":"e_1_3_2_70_2","article-title":"Determination of minimum sample size requirement for multiple linear regression and analysis of covariance based on experimental and non\u2010experimental studies","volume":"14","author":"Bujang MA","year":"2017","unstructured":"Bujang MA, Baharum N. Determination of minimum sample size requirement for multiple linear regression and analysis of covariance based on experimental and non\u2010experimental studies. Epidemiol Biostat Public Health. 2017;14:e12267\u20101\u201010.","journal-title":"Epidemiol Biostat Public Health"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.120.018322","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:14:11Z","timestamp":1715598851000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.120.018322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,16]]},"references-count":69,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,3,16]]}},"alternative-id":["10.1161\/JAHA.120.018322"],"URL":"https:\/\/doi.org\/10.1161\/jaha.120.018322","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,16]]},"assertion":[{"value":"2020-07-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e018322"}}