{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:34:29Z","timestamp":1792722869536,"version":"4.5.0"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2021,1,5]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Release of neutrophil extracellular traps (NETs) after percutaneous coronary intervention (PCI) in acute coronary syndrome (ACS) is associated with periprocedural myocardial infarction, as a result of microvascular obstruction via pro\u2010inflammatory and prothrombotic pathways. Colchicine is a well\u2010established anti\u2010inflammatory agent with growing evidence to support use in patients with coronary disease. However, its effects on post\u2010PCI NET formation in ACS have not been explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Sixty patients (40 ACS; 20 stable angina pectoris) were prospectively recruited and allocated to colchicine or no treatment. Within 24\u00a0hours of treatment, serial coronary sinus blood samples were collected during PCI. Isolated neutrophils from 10 patients with ACS post\u2010PCI and 4 healthy controls were treated in vitro with colchicine (25\u00a0nmol\/L) and stimulated with either ionomycin (5\u00a0\u03bcmol\/L) or phorbol 12\u2010myristate 13\u2010acetate (50\u00a0nmol\/L). Extracellular DNA was quantified using Sytox Green and fixed cells were stained with Hoechst 3342 and anti\u2010alpha tubulin. Baseline characteristics were similar across both treatment and control arms. Patients with ACS had higher NET release versus patients with stable angina pectoris (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.001), which was reduced with colchicine treatment (area under the curve: 0.58 versus 4.29;\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.001). In vitro, colchicine suppressed unstimulated (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.001), phorbol 12\u2010myristate 13\u2010acetate\u2013induced (\n                      <jats:italic>P<\/jats:italic>\n                      =0.009) and ionomycin\u2010induced (\n                      <jats:italic>P<\/jats:italic>\n                      =0.002) NET formation in neutrophils isolated from patients with ACS post\u2010PCI, but not healthy controls. Tubulin organization was impaired in neutrophils from patients with ACS but was restored by colchicine treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Colchicine suppresses NET formation in patients with ACS post\u2010PCI by restoring cytoskeletal dynamics. These findings warrant further investigation in randomized trials powered for clinical end points.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Registration<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      URL:\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/anzctr.org.au\">https:\/\/anzctr.org.au<\/jats:ext-link>\n                      ; Unique identifier: ACTRN12619001231134.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.120.018993","type":"journal-article","created":{"date-parts":[[2020,12,21]],"date-time":"2020-12-21T08:34:44Z","timestamp":1608539684000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":130,"title":["Colchicine Inhibits Neutrophil Extracellular Trap Formation in Patients With Acute Coronary Syndrome After Percutaneous Coronary Intervention"],"prefix":"10.1161","volume":"10","author":[{"given":"Kaivan","family":"Vaidya","sequence":"first","affiliation":[{"name":"Royal Prince Alfred Hospital Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7028-4331","authenticated-orcid":false,"given":"Bradley","family":"Tucker","sequence":"additional","affiliation":[{"name":"Heart Research Institute Sydney Australia"},{"name":"School of Medical Sciences UNSW Sydney Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2909-4296","authenticated-orcid":false,"given":"Rahul","family":"Kurup","sequence":"additional","affiliation":[{"name":"Royal Prince Alfred Hospital Sydney Australia"},{"name":"Heart Research Institute Sydney 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