{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:35:27Z","timestamp":1791920127680,"version":"4.3.4"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Animal disease models represent the cornerstone in basic cardiac arrest (CA) research. However, current experimental models of CA and resuscitation in mice are limited. In this study, we aimed to develop a mouse model of asphyxial CA followed by cardiopulmonary resuscitation (CPR), and to characterize the immune response after asphyxial CA\/CPR.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              CA was induced in mice by switching from an O\n              <jats:sub>2<\/jats:sub>\n              \/N\n              <jats:sub>2<\/jats:sub>\n              mixture to 100% N\n              <jats:sub>2<\/jats:sub>\n              gas for mechanical ventilation under anesthesia. Real\u2010time measurements of blood pressure, brain tissue oxygen, cerebral blood flow, and ECG confirmed asphyxia and ensuing CA. After a defined CA period, mice were resuscitated with intravenous epinephrine administration and chest compression. We subjected young adult and aged mice to this model, and found that after CA\/CPR, mice from both groups exhibited significant neurologic deficits compared with sham mice. Analysis of post\u2010CA brain confirmed neuroinflammation. Detailed characterization of the post\u2010CA immune response in the peripheral organs of both young adult and aged mice revealed that at the subacute phase following asphyxial CA\/CPR, the immune system was markedly suppressed as manifested by drastic atrophy of the spleen and thymus, and profound lymphopenia. Finally, our data showed that post\u2010CA systemic lymphopenia was accompanied with impaired T and B lymphopoiesis in the thymus and bone marrow, respectively.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">In this study, we established a novel validated asphyxial CA model in mice. Using this new model, we further demonstrated that asphyxial CA\/CPR markedly affects both the nervous and immune systems, and notably impairs lymphopoiesis of T and B cells.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.120.019142","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T09:12:34Z","timestamp":1621501954000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["Development and Evaluation of a Novel Mouse Model of Asphyxial Cardiac Arrest Revealed Severely Impaired Lymphopoiesis After Resuscitation"],"prefix":"10.1161","volume":"10","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Center for Perioperative Organ Protection Duke University Medical Center Durham NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Li","sequence":"additional","affiliation":[{"name":"Department 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