{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T19:20:35Z","timestamp":1783624835930,"version":"3.55.0"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Saphenous vein grafts (SVGs) are broadly used in coronary artery bypass grafting despite their inferior patency compared with arterial grafts. Recently, the no\u2010touch technique (NT), in which an SVG is harvested with a pedicle of perivascular adipose tissue (PVAT) without conduit distension, was shown to improve long\u2010term patency compared with conventional preparation (CV), wherein outer tissue is removed with distension. The NT was also reportedly associated with reduced atherosclerosis. Although endothelial damage provoked by conventional distension may underlie poor patency when CV is performed, the precise mechanisms underlying the salutary effects of the NT have been unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Residual SVGs prepared with CV (CV\u2010SVGs) or NT (NT\u2010SVGs) were obtained during coronary artery bypass grafting. Nitric oxide (NO\n              <jats:sub>2<\/jats:sub>\n              <jats:sup>\u2212<\/jats:sup>\n              \/NO\n              <jats:sub>3<\/jats:sub>\n              <jats:sup>\u2212<\/jats:sup>\n              (NO\n              <jats:sub>x<\/jats:sub>\n              )) levels after 24\u00a0hours of tissue culture were quantified. The protein expression and localization were analyzed. The isometric force of SVG strips was measured. NT\u2010SVGs showed superior NO\n              <jats:sub>x<\/jats:sub>\n              production to CV\u2010SVGs. PVAT generated the majority of NO\n              <jats:sub>x<\/jats:sub>\n              in NT\u2010SVGs. PVAT highly expressed arginosuccinate synthase 1, a rate\u2010limiting enzyme in the molecular circuit for NO synthesis, thereby continuously providing the substrate for NO. A substantial level of endothelial NO synthase was also expressed in PVAT. Pharmacological inhibition of arginosuccinate synthase 1 or endothelial NO synthase significantly suppressed the NO\n              <jats:sub>x<\/jats:sub>\n              production in NT\u2010SVGs. PVAT induced vasorelaxation through NO production, even in the endothelium\u2010denuded SVG strips.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Preserving PVAT was predominantly involved in the superior NO\n              <jats:sub>x<\/jats:sub>\n              production in NT\u2010SVGs. Since NO plays crucial roles in suppressing atherosclerosis, this mechanism may greatly contribute to the excellent patency in NT\u2010SVGs.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.120.020637","type":"journal-article","created":{"date-parts":[[2022,1,19]],"date-time":"2022-01-19T10:37:45Z","timestamp":1642588665000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":38,"title":["Perivascular Adipose Tissue Is a Major Source of Nitric Oxide in Saphenous Vein Grafts Harvested via the No\u2010Touch Technique"],"prefix":"10.1161","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0845-9265","authenticated-orcid":false,"given":"Toshiro","family":"Saito","sequence":"first","affiliation":[{"name":"Department of Surgery and Clinical Science Yamaguchi University Graduate School of Medicine Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4009-7391","authenticated-orcid":false,"given":"Hiroshi","family":"Kurazumi","sequence":"additional","affiliation":[{"name":"Department of Surgery and Clinical Science Yamaguchi University Graduate School of Medicine Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5465-5948","authenticated-orcid":false,"given":"Ryo","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Surgery and Clinical Science Yamaguchi University Graduate School of Medicine Yamaguchi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9474-6898","authenticated-orcid":false,"given":"Kazumasa","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Surgery and Clinical Science Yamaguchi University Graduate School of Medicine Yamaguchi 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