{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:36:13Z","timestamp":1789882573104,"version":"4.0.1"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"13","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2021,7,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Overweight adults have low circulating concentrations of ANP (atrial natriuretic peptide) and proANP fragments. We tested the hypothesis that an intensive lifestyle intervention with an intended weight loss would increase plasma concentrations of a proANP fragment in overweight children.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              We measured MR\u2010proANP (midregional proANP) concentrations in plasma from overweight children who participated in the OOIS (Odense Overweight Intervention Study). OOIS randomized 115 overweight children (11\u201313\u00a0years, 55% girls) to an intensive day\u2010camp intervention arm with increased physical activity and healthy diet or to a less intensive standard intervention arm for 6\u00a0weeks. We used linear mixed\u2010effects modeling for repeated measures to estimate the difference in the mean change with 95% CIs in fasting plasma MR\u2010proANP concentrations between the 2 arms, and we used partial least squares regression analysis to identify candidate mediators. Differences in weight, fitness, and metabolic factors were also analyzed. At baseline, fasting plasma MR\u2010proANP concentrations were (median [interquartile range]) 35.0\u00a0pmol\/L (26.8\u201342.0) in the day\u2010camp intervention arm and 37.2\u00a0pmol\/L (31.7\u201344.7) in standard intervention arm participants, respectively. After 6\u00a0weeks intervention, children in the day\u2010camp intervention arm had increased their MR\u2010proANP (5.4\u00a0pmol\/L [0.8\u201310.0],\n              <jats:italic>P<\/jats:italic>\n              =0.022) and their fitness (2.33\u00a0mL O\n              <jats:sub>2<\/jats:sub>\n              \/min per kg [0.52\u20134.14],\n              <jats:italic>P<\/jats:italic>\n              =0.012) and they had deceased their body mass index (\u22122.12\u00a0kg\/m\n              <jats:sup>2<\/jats:sup>\n              [\u22122.59 to \u22121.65],\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001) as compared with children in standard intervention arm. In the partial least squares analysis, decreases in fasting insulin and in estimated insulin resistance were associated with the observed increase in MR\u2010proANP concentrations.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">An intensive lifestyle intervention increases plasma MR\u2010proANP among overweight children.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Registration<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              URL:\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/www.clinicaltrials.gov\">https:\/\/www.clinicaltrials.gov<\/jats:ext-link>\n              ; Unique identifier: NCT01574352.\n            <\/jats:p>\n          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Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3918-6645","authenticated-orcid":false,"given":"Kristian T.","family":"Larsen","sequence":"additional","affiliation":[{"name":"Center of Research in Childhood Health (RICH) Department of Sports Science and Clinical Biomechanics University of Southern Denmark Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens P.","family":"Goetze","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry Rigshospitalet BlegdamsvejUniversity of Copenhagen Denmark"},{"name":"Department of Biomedical Sciences Faculty of Health and Medical Sciences University of Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9800-029X","authenticated-orcid":false,"given":"Lars B.","family":"Andersen","sequence":"additional","affiliation":[{"name":"Department of Sport, Food, and Natural Sciences Campus SogndalWestern Norway University of Applied Sciences, Sogndal Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4320-8015","authenticated-orcid":false,"given":"J\u00f8rgen L.","family":"Jeppesen","sequence":"additional","affiliation":[{"name":"Department of Medicine Amager Hvidovre Hospital in GlostrupUniversity of Copenhagen Glostrup Denmark"},{"name":"Department of Clinical Medicine Faculty of Health and Medical Sciences University of Copenhagen 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