{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:40:15Z","timestamp":1788381615890,"version":"build-2803163510"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2021,8,3]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Placental derived cell\u2010free DNA (cfDNA), widely utilized for prenatal screening, may serve as a biomarker for preeclampsia. To determine whether cfDNA parameters are altered in preeclampsia, we conducted a case\u2010control study using prospectively collected maternal plasma (n=20 preeclampsia, n=22 normal) using our in\u2010house validated prenatal screening assay.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Isolated cfDNA was quantified, sequenced using Illumina NextSeq 500, and the placental\u2010derived fraction was determined. Clinical and test characteristics were compared between preeclampsia and controls, followed by comparisons within the preeclampsia cohort dichotomized by cfDNA concentration. Lastly, cfDNA parameters in preeclampsia were correlated with markers of disease severity. Maternal age, body mass index, gestational age at delivery, cesarean rate, and neonatal birthweight were expectedly different between groups (\n              <jats:italic>P<\/jats:italic>\n              \u22640.05). The placental\u2010derived cfDNA fraction did not differ between groups (21.4% versus 16.9%,\n              <jats:italic>P<\/jats:italic>\n              =0.06); however, total cfDNA was more than 10 times higher in preeclampsia (1235 versus 106.5\u00a0pg\/\u00b5L,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001). This relationship persisted when controlling for important confounders (OR 1.22, 95% CI 1.04\u20131.43,\n              <jats:italic>P<\/jats:italic>\n              =0.01). The dichotomized preeclampsia group with the highest cfDNA concentration delivered earlier (33.2 versus 36.6\u00a0weeks,\n              <jats:italic>P<\/jats:italic>\n              =0.02) and had lower placental\u2010derived fractions (9.1% versus 21.4%,\n              <jats:italic>P<\/jats:italic>\n              =0.04). Among preeclampsia cases, higher total cfDNA correlated with earlier gestational age at delivery (\n              <jats:italic>P<\/jats:italic>\n              =0.01) and higher maximum systolic blood pressure (\n              <jats:italic>P<\/jats:italic>\n              =0.04).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">At diagnosis, total cfDNA is notably higher in preeclampsia, whereas the placental derived fraction remains similar to healthy pregnancies. In preeclampsia, higher total cfDNA correlates with earlier gestational age at delivery and higher systolic blood pressure. These findings may indicate increased release of cfDNA from maternal tissue injury.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.121.021477","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T16:20:02Z","timestamp":1627316402000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["At Preeclampsia Diagnosis, Total Cell\u2010Free DNA Concentration is Elevated and Correlates With Disease Severity"],"prefix":"10.1161","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8093-0774","authenticated-orcid":false,"given":"Teodora R.","family":"Kolarova","sequence":"first","affiliation":[{"name":"Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary S.","family":"Gammill","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Lee","family":"Nelson","sequence":"additional","affiliation":[{"name":"Clinical Research Division Fred Hutchinson Cancer Research Center Seattle WA"},{"name":"Division of Rheumatology Department of Medicine University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Lockwood","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2747-2097","authenticated-orcid":false,"given":"Raj","family":"Shree","sequence":"additional","affiliation":[{"name":"Division of Maternal Fetal Medicine Department of Obstetrics and Gynecology University of Washington Seattle WA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1407349"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1667374"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/uog.12331"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2019.04.002"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14671"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4416"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.05.025"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.2.184"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.109594"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200360"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.12891\/ceog1982.2015"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01486"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.15432"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000003018"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406103111"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.4.650"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-120"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.l2381"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13293-020-00299-3"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.4625"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000002752"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2019.1598759"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.11.050"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.242719.118"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2003.12.019"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2015.03.026"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29766"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000002208"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.943407"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000053441.43495.1A"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.121.021477","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:47:19Z","timestamp":1715600839000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.121.021477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,3]]},"references-count":31,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2021,8,3]]}},"alternative-id":["10.1161\/JAHA.121.021477"],"URL":"https:\/\/doi.org\/10.1161\/jaha.121.021477","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,3]]},"assertion":[{"value":"2021-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e021477"}}