{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:06:03Z","timestamp":1790445963838,"version":"4.1.0"},"reference-count":68,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2022,1,4]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">In the myocardium, pericytes are often confused with other interstitial cell types, such as fibroblasts. The lack of well\u2010characterized and specific tools for identification, lineage tracing, and conditional targeting of myocardial pericytes has hampered studies on their role in heart disease. In the current study, we characterize and validate specific and reliable strategies for labeling and targeting of cardiac pericytes.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Using the neuron\u2010glial antigen 2 (NG2)\n              <jats:sup>DsRed<\/jats:sup>\n              reporter line, we identified a large population of NG2+ periendothelial cells in mouse atria, ventricles, and valves. To examine possible overlap of NG2+ mural cells with fibroblasts, we generated NG2\n              <jats:sup>DsRed<\/jats:sup>\n              ; platelet\u2010derived growth factor receptor (PDGFR) \u03b1\n              <jats:sup>EGFP<\/jats:sup>\n              pericyte\/fibroblast dual reporter mice. Myocardial NG2+ pericytes and PDGFR\u03b1+ fibroblasts were identified as nonoverlapping cellular populations with distinct transcriptional signatures. PDGFR\u03b1+ fibroblasts expressed high levels of fibrillar collagens, matrix metalloproteinases, tissue inhibitor of metalloproteinases, and genes encoding matricellular proteins, whereas NG2+ pericytes expressed high levels of\n              <jats:italic>Pdgfrb<\/jats:italic>\n              ,\n              <jats:italic>Adamts1<\/jats:italic>\n              , and\n              <jats:italic>Vtn<\/jats:italic>\n              . To validate the specificity of pericyte Cre drivers, we crossed these lines with PDGFR\u03b1\n              <jats:sup>EGFP<\/jats:sup>\n              fibroblast reporter mice. The constitutive NG2\n              <jats:sup>Cre<\/jats:sup>\n              driver did not specifically track mural cells, labeling many cardiomyocytes. However, the inducible NG2\n              <jats:sup>CreER<\/jats:sup>\n              driver specifically traced vascular mural cells in the ventricle and in the aorta, without significant labeling of PDGFR\u03b1+ fibroblasts. In contrast, the inducible PDGFR\u03b2\n              <jats:sup>CreER<\/jats:sup>\n              line labeled not only mural cells but also the majority of cardiac and aortic fibroblasts.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Fibroblasts and pericytes are topographically and transcriptomically distinct populations of cardiac interstitial cells. The inducible NG2\n              <jats:sup>CreER<\/jats:sup>\n              driver optimally targets cardiac pericytes; in contrast, the inducible PDGFR\u03b2\n              <jats:sup>CreER<\/jats:sup>\n              line lacks specificity.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.121.023171","type":"journal-article","created":{"date-parts":[[2021,12,22]],"date-time":"2021-12-22T11:50:55Z","timestamp":1640173855000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["Validation of Specific and Reliable Genetic Tools to Identify, Label, and Target Cardiac Pericytes in Mice"],"prefix":"10.1161","volume":"11","author":[{"given":"Linda","family":"Alex","sequence":"first","affiliation":[{"name":"Department of Medicine (Cardiology) The Wilf Family Cardiovascular Research InstituteAlbert Einstein College of Medicine Bronx 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