{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T09:47:54Z","timestamp":1789984074455,"version":"4.0.1"},"reference-count":52,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2023,2,21]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Therapeutic hypothermia has a beneficial effect on cardiac function after acute myocardial infarction, but the exact mechanism is still unclear. Recent research has suggested that microRNAs participate in acute myocardial infarction to regulate cardiomyocyte survival. This study aimed to explore the ability of hypothermia\u2010regulated microRNA\u2010483\u20103p (miR\u2010483\u20103p) to inhibit hypoxia\u2010induced myocardial infarction.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Primary cardiomyocytes were cultured under hypoxia at 32 \u00b0C to mimic therapeutic hypothermia, and the differentially expressed microRNAs were determined by RNA sequencing. Therapeutic hypothermia recovered hypoxia\u2010induced increases in apoptosis, decreases in ATP levels, and decreases in miR\u2010483\u20103p expression. Overexpression of miR\u2010483\u20103p exhibited effects similar to those of therapeutic hypothermia on hypoxia in the treatment of cardiomyocytes to associate with maintaining the mitochondrial membrane potential, and cyclin\u2010dependent kinase 9 (Cdk9) was identified as a target gene with downregulated expression by miR\u2010483\u20103p. Knockdown of Cdk9 also promoted cardiac survival, ATP production, and mitochondrial membrane potential stability under hypoxia. In vivo, the expression of miR\u2010483\u20103p and Cdk9 was tested in the cardiac tissue of the mice with acute myocardial infarction, and the expression of miR\u2010483\u20103p decreased and Cdk9 increased in the region of myocardial infarction. However, miR\u2010483\u20103p was overexpressed with lentivirus, which suppressed apoptosis, infarct size (miR\u2010483\u20103p,\u00a022.00\u00b14.04% versus negative control,\u00a028.57\u00b15.44%,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05), and Cdk9 expression to improve cardiac contractility.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">MiR\u2010483\u20103p antagonizes hypoxia, leading to cardiomyocyte injury by targeting Cdk9, which is a new mechanism of therapeutic hypothermia.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.122.026160","type":"journal-article","created":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T12:16:51Z","timestamp":1676981811000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Therapeutic Hypothermia Inhibits Hypoxia\u2010Induced Cardiomyocyte Apoptosis\u00a0Via the MiR\u2010483\u20103p\/Cdk9 Axis"],"prefix":"10.1161","volume":"12","author":[{"given":"Qiqi","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Geriatrics Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianru","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology Shanghai Ninth People\u2019s Hospital, Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cardiac Imaging 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