{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T04:57:07Z","timestamp":1768712227124,"version":"3.49.0"},"reference-count":62,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"21","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\"><jats:title>Background<\/jats:title><jats:p xml:lang=\"en\">Restenosis is one of the main bottlenecks in restricting the further development of cardiovascular interventional therapy. New signaling molecules involved in the progress have continuously been discovered; however, the specific molecular mechanisms remain unclear. MTMR14 (myotubularin\u2010related protein 14) is a novel phosphoinositide phosphatase that has a variety of biological functions and is involved in diverse biological processes. However, the role of MTMR14 in vascular biology remains unclear. Herein, we addressed the role of MTMR14 in neointima formation and vascular smooth muscle cell (VSMC) proliferation after vessel injury.<\/jats:p><\/jats:sec><jats:sec xml:lang=\"en\"><jats:title>Methods and Results<\/jats:title><jats:p xml:lang=\"en\">Vessel injury models were established using SMC\u2010specific conditional MTMR14\u2010knockout and \u2010transgenic mice. Neointima formation was assessed by histopathological methods, and VSMC proliferation and migration were assessed using fluorescence ubiquitination\u2010based cell cycle indicator, transwell, and scratch wound assay. Neointima formation and the expression of MTMR14 was increased after injury. MTMR14 deficiency accelerated neointima formation and promoted VSMC proliferation after injury, whereas MTMR14 overexpression remarkably attenuated this process. Mechanistically, we demonstrated that MTMR14 suppressed the activation of PLK1 (polo\u2010like kinase 1) by interacting with it, which further leads to the inhibition of the activation of MEK\/ERK\/AKT (mitogen\u2010activated protein kinase kinase\/extracellular\u2010signal\u2010regulated kinase\/protein kinase B), thereby inhibiting the proliferation of VSMC from the medial to the intima and thus preventing neointima formation.<\/jats:p><\/jats:sec><jats:sec xml:lang=\"en\"><jats:title>Conclusions<\/jats:title><jats:p xml:lang=\"en\">MTMR14 prevents neointima formation and VSMC proliferation by inhibiting PLK1. Our findings reveal that MTMR14 serves as an inhibitor of VSMC proliferation and establish a link between MTMR14 and PLK1 in regulating VSMC proliferation. MTMR14 may become a novel potential therapeutic target in the treatment of restenosis.<\/jats:p><\/jats:sec>","DOI":"10.1161\/jaha.122.026174","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T10:48:30Z","timestamp":1667213310000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Myotubularin\u2010Related Protein14 Prevents Neointima Formation and Vascular Smooth Muscle Cell Proliferation by Inhibiting Polo\u2010Like Kinase1"],"prefix":"10.1161","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8279-1827","authenticated-orcid":false,"given":"Ling\u2010Yao","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou University Zhengzhou 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7th People\u2019s Hospital of Zhengzhou Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dian\u2010Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u2010Lu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of Zhengzhou University Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng\u2010Yang","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Cardiology, 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