{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:45:37Z","timestamp":1784875537419,"version":"3.55.0"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2023,3,21]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Mitochondrial abnormalities exist in gastrocnemius muscle of people with peripheral artery disease (PAD). Whether abnormalities in mitochondrial biogenesis and autophagy are associated with greater ischemia or walking impairment in PAD is unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Protein markers of mitochondrial biogenesis and autophagy and the abundance of mitochondrial electron transport chain complexes were quantified in gastrocnemius muscle biopsies from people with and without PAD. Their 6\u2010minute walk distance and 4\u2010m gait speed were measured. Sixty\u2010seven participants (mean age 65.0\u2009years [\u00b16.8], 16 [23.9%] women, 48 [71.6%] Black) were enrolled, including 15 with moderate to severe PAD (ankle brachial index [ABI] &lt;0.60), 29 with mild PAD (ABI 0.60\u20130.90), and 23 without PAD (ABI 1.00\u20131.40). Abundance of all electron transport chain complexes was significantly higher in participants with lower ABI (eg, complex I: 0.66, 0.45, 0.48 arbitrary units [AU], respectively,\n              <jats:italic>P<\/jats:italic>\n              trend=0.043). Lower ABI values were associated with a higher LC3A\/B II\u2010to\u2010LC3A\/B I (microtubule\u2010associated protein 1A\/1B\u2010light chain 3) ratio (2.54, 2.31, 2.15 AU, respectively,\n              <jats:italic>P<\/jats:italic>\n              trend=0.017) and reduced abundance of the autophagy receptor p62 (0.71, 0.69, 0.80 AU, respectively,\n              <jats:italic>P<\/jats:italic>\n              trend=0.033). The abundance of each electron transport chain complex was positively and significantly associated with 6\u2010minute walk distance and 4\u2010m gait speed at usual and fast pace only among participants without PAD (eg, complex I:\n              <jats:italic>r<\/jats:italic>\n              =0.541,\n              <jats:italic>P<\/jats:italic>\n              =0.008;\n              <jats:italic>r<\/jats:italic>\n              =0.477,\n              <jats:italic>P<\/jats:italic>\n              =0.021;\n              <jats:italic>r<\/jats:italic>\n              =0.628,\n              <jats:italic>P<\/jats:italic>\n              =0.001, respectively).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">These results suggest that accumulation of electron transport chain complexes in gastrocnemius muscle of people with PAD may be because of impaired mitophagy in the setting of ischemia. Findings are descriptive, and further study in larger sample sizes is needed.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.122.027088","type":"journal-article","created":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T18:21:24Z","timestamp":1682619684000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Mitochondrial Complex Abundance, Mitophagy Proteins, and Physical Performance in People With and Without Peripheral Artery Disease"],"prefix":"10.1161","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7032-3487","authenticated-orcid":false,"given":"Anna","family":"Picca","sequence":"first","affiliation":[{"name":"Fondazione Policlinico Universitario \"Agostino Gemelli\" IRCCS Rome Italy"},{"name":"Department of Medicine and Surgery LUM University Casamassima 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All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7534-0497","authenticated-orcid":false,"given":"Sudarshan","family":"Dayanidhi","sequence":"additional","affiliation":[{"name":"Departments of Physical Medicine and Rehabilitation, Physical Therapy and Human Movement Science Northwestern University, Feinberg School of Medicine Chicago IL USA"},{"name":"Shirley Ryan AbilityLab Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2912-2020","authenticated-orcid":false,"given":"Dongxue","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine Northwestern University, Feinberg School of Medicine Chicago IL 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