{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:34:59Z","timestamp":1790836499996,"version":"4.1.0"},"reference-count":25,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2023,1,17]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Mechanisms underlying bioprosthetic valve deterioration are multifactorial and incompletely elucidated. Reparative circulating progenitor cells, and conversely calcification\u2010associated osteocalcin expressing circulating progenitor cells, have been linked to native aortic valve deterioration. However, their role in bioprosthetic valve deterioration remains elusive. This study sought to evaluate the contribution of different subpopulations of circulating progenitor cells in bioprosthetic valve deterioration.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              This single\u2010center prospective study enrolled 121 patients who had peripheral blood mononuclear cells isolated before bioprosthetic aortic valve replacement and had an echocardiographic follow\u2010up \u22652\u2009years after the procedure. Using flow cytometry, fresh peripheral blood mononuclear cells were analyzed for the surface markers CD34, CD133, and osteocalcin. Bioprosthetic valve deterioration was evaluated by hemodynamic valve deterioration (HVD) using echocardiography, which was defined as an elevated mean transprosthetic gradient \u226530\u2009mm\u2009Hg or at least moderate intraprosthetic regurgitation. Sixteen patients (13.2%) developed HVD during follow\u2010up for a median of 5.9\u2009years. Patients with HVD showed significantly lower levels of reparative CD34\n              <jats:sup>+<\/jats:sup>\n              CD133\n              <jats:sup>+<\/jats:sup>\n              cells and higher levels of osteocalcin\u2010positive cells than those without HVD (CD34\n              <jats:sup>+<\/jats:sup>\n              CD133\n              <jats:sup>+<\/jats:sup>\n              cells: 125 [80, 210] versus 270 [130, 420],\n              <jats:italic>P<\/jats:italic>\n              =0.002; osteocalcin\u2010positive cells: 3060 [523, 5528] versus 670 [180, 1930],\n              <jats:italic>P<\/jats:italic>\n              =0.005 respectively). Decreased level of CD34\n              <jats:sup>+<\/jats:sup>\n              CD133\n              <jats:sup>+<\/jats:sup>\n              cells was a significant predictor of HVD (hazard ratio, 0.995 [95% CI, 0.990%\u20130.999%]).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Circulating levels of CD34\n              <jats:sup>+<\/jats:sup>\n              CD133\n              <jats:sup>+<\/jats:sup>\n              cells and osteocalcin\u2010positive cells were significantly associated with the subsequent occurrence of HVD in patients undergoing bioprosthetic aortic valve replacement. Circulating progenitor cells might play a vital role in the mechanism, risk stratification, and a potential therapeutic target for patients with bioprosthetic valve deterioration.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.122.027364","type":"journal-article","created":{"date-parts":[[2023,1,16]],"date-time":"2023-01-16T11:18:06Z","timestamp":1673867886000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Circulating Progenitor Cells Are Associated With Bioprosthetic Aortic Valve Deterioration: A Preliminary Study"],"prefix":"10.1161","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6535-4377","authenticated-orcid":false,"given":"Yoshihisa","family":"Kanaji","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"},{"name":"Division of Cardiovascular Medicine Tsuchiura Kyodo General Hospital Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2006-3431","authenticated-orcid":false,"given":"Ilke","family":"Ozcan","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4681-2798","authenticated-orcid":false,"given":"Takumi","family":"Toya","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"},{"name":"Division of Cardiology National Defense Medical College Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2713-0433","authenticated-orcid":false,"given":"Rajiv","family":"Gulati","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4114-7768","authenticated-orcid":false,"given":"Melissa","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4004-5292","authenticated-orcid":false,"given":"Tsunekazu","family":"Kakuta","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine Tsuchiura Kyodo General Hospital Ibaraki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3271-3887","authenticated-orcid":false,"given":"Lilach O.","family":"Lerman","sequence":"additional","affiliation":[{"name":"Division of Nephrology and Hypertension, Mayo Clinic Mayo Clinic Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-5313","authenticated-orcid":false,"given":"Amir","family":"Lerman","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Rochester MN"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.12679"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/HCO.0000000000000372"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.07.042"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.01.005"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/eht034"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2008.07.019"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab203"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0249187"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2014.10.003"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2019.01.027"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx303"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2019.05.016"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2009.03.075"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2014.10.005"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehi458"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2009.09.016"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1661"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.10.3106"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.018921"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207434"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.005648"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.304187"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aau6934"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.319644"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCINTERVENTIONS.121.010802"}],"container-title":["Journal 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