{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:48:52Z","timestamp":1775058532192,"version":"3.50.1"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"13","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2023,7,4]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Appropriate treatment of pulmonary hypertension (PH) is critically dependent on accurate discrimination between pre\u2010 and postcapillary PH. However, clinical discrimination is challenging and frequently requires a right heart catheterization. Existing risk scores to detect postcapillary PH have suboptimal discriminatory strength. We have previously shown that platelet\u2010derived RNA profiles may have diagnostic value for PH detection. Here, we hypothesize that platelet\u2010derived RNAs can be employed to select unique biomarker panels for the discrimination between pre\u2010 and postcapillary PH.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">Blood platelet RNA from whole blood was isolated and sequenced from 50 patients with precapillary PH (with different PH subtypes) as well as 50 patients with postcapillary PH. RNA panels were calculated by ANOVA statistics, and classifications were performed using a support vector machine algorithm, supported by particle swarm optimization. We identified in total 4279 different RNAs in blood platelets from patients with pre\u2010 and postcapillary PH. A particle swarm optimization\u2013selected RNA panel of 1618 distinctive RNAs with differential levels together with a trained support vector machine algorithm accurately discriminated patients with precapillary PH from patients with postcapillary PH with 100% sensitivity, 60% specificity, 80% accuracy, and 0.95 (95% CI, 0.86\u20131.00) area under the curve in the independent validation series (n=20).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">This proof\u2010of\u2010concept study demonstrates that particle swarm optimization\/support vector machine\u2013enhanced classification of platelet RNA panels may be able to discriminate precapillary PH from postcapillary PH. This research provides a foundation for the development of a blood test with a high negative predictive value that would improve early diagnosis of precapillary PH and prevents unnecessary invasive testing in patients with postcapillary PH.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.122.028447","type":"journal-article","created":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T13:00:15Z","timestamp":1687438815000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Discrimination Between Pre\u2010 and Postcapillary Pulmonary Hypertension Using Platelet RNA"],"prefix":"10.1161","volume":"12","author":[{"given":"Mohammad","family":"Arkani","sequence":"first","affiliation":[{"name":"Amsterdam Cardiovascular Sciences Pulmonary Hypertension and Thrombosis Amsterdam The Netherlands"},{"name":"Department of Pulmonology Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Department of Neurosurgery Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam Brain Tumor Center Amsterdam Amsterdam The Netherlands"},{"name":"Department of Biomedical Data Sciences Leiden University Medical Center Leiden The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1238-4139","authenticated-orcid":false,"given":"Azar","family":"Kianzad","sequence":"additional","affiliation":[{"name":"Amsterdam Cardiovascular Sciences Pulmonary Hypertension and Thrombosis Amsterdam The Netherlands"},{"name":"Department of Pulmonology Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3493-3573","authenticated-orcid":false,"given":"Samara","family":"Jansen","sequence":"additional","affiliation":[{"name":"Amsterdam Cardiovascular Sciences Pulmonary Hypertension and Thrombosis Amsterdam The Netherlands"},{"name":"Department of Pulmonology Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9828-0407","authenticated-orcid":false,"given":"A. 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G.","family":"In\u2019t Veld","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam Brain Tumor Center Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5992-4468","authenticated-orcid":false,"given":"David P.","family":"Noske","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam Brain Tumor Center Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4057-758X","authenticated-orcid":false,"given":"Anton","family":"Vonk Noordegraaf","sequence":"additional","affiliation":[{"name":"Amsterdam Cardiovascular Sciences Pulmonary Hypertension and Thrombosis Amsterdam The Netherlands"},{"name":"Department of Pulmonology Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Wurdinger","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam Brain Tumor Center Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myron G.","family":"Best","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"},{"name":"Cancer Center Amsterdam Brain Tumor Center Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5371-0346","authenticated-orcid":false,"given":"Harm\u2010Jan","family":"Bogaard","sequence":"additional","affiliation":[{"name":"Amsterdam Cardiovascular Sciences Pulmonary Hypertension and Thrombosis Amsterdam The Netherlands"},{"name":"Department of Pulmonology Amsterdam UMC Location Vrije Universiteit Amsterdam Amsterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/EURHEARTJ\/EHV317"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/JAMAINTERNMED.2013.319"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.034646"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00202814"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.119.015992"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389201018666170615082510"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.121.056719"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.202201-085OC"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2005.06.015"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/BLOOD-2018-12-852830"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0139-5"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.07.004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1586\/ERS.11.17"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1152\/AJPHEART.00136.2018"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.102.20.2460"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1041"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/JCLA.24273"},{"key":"e_1_3_2_19_2","article-title":"Confidence intervals for the area under the ROC curve","volume":"17","author":"Cortes C","year":"2004","unstructured":"Cortes C, Mohri M. 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