{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:04:29Z","timestamp":1790816669983,"version":"4.1.0"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2023,6,6]]},"abstract":"<jats:sec id=\"jah38467-sec-0001\" sec-type=\"background\" xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Normal brain function depends on the ability of the vasculature to increase blood flow to regions with high metabolic demands. Impaired neurovascular coupling, such as the local hyperemic response to neuronal activity, may contribute to poor neurological outcome after stroke despite successful recanalization, that is, futile recanalization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"jah38467-sec-0002\" xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Mice implanted with chronic cranial windows were trained for awake head\u2010fixation before experiments. One\u2010hour occlusion of the anterior middle cerebral artery branch was induced using single\u2010vessel photothrombosis. Cerebral perfusion and neurovascular coupling were assessed by optical coherence tomography and laser speckle contrast imaging. Capillaries and pericytes were studied in perfusion\u2010fixed tissue by labeling lectin and platelet\u2010derived growth factor receptor \u03b2. Arterial occlusion induced multiple spreading depolarizations over 1\u2009hour associated with substantially reduced blood flow in the peri\u2010ischemic cortex. Approximately half of the capillaries in the peri\u2010ischemic area were no longer perfused at the 3\u2010 and 24\u2010hour follow\u2010up (45% [95% CI, 33%\u201358%] and 53% [95% CI, 39%\u201366%] reduction, respectively;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.0001), which was associated with contraction of an equivalent proportion of peri\u2010ischemic capillary pericytes. The capillaries in the peri\u2010ischemic cortex that remained perfused showed increased point prevalence of dynamic flow stalling (0.5% [95% CI, 0.2%\u20130.7%] at baseline, 5.1% [95% CI, 3.2%\u20136.5%] and 3.2% [95% CI, 1.1%\u20135.3%] at 3\u2010 and 24\u2010hour follow\u2010up, respectively;\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.001). Whisker stimulation at the 3\u2010 and 24\u2010hour follow\u2010up led to reduced neurovascular coupling responses in the sensory cortex corresponding to the peri\u2010ischemic region compared with that observed at baseline.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"jah38467-sec-0003\" xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Arterial occlusion led to contraction of capillary pericytes and capillary flow stalling in the peri\u2010ischemic cortex. Capillary dysfunction was associated with neurovascular uncoupling. Neurovascular coupling impairment associated with capillary dysfunction may be a mechanism that contributes to futile recanalization. Hence, the results from this study suggest a novel treatment target to improve neurological outcome after stroke.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.123.029527","type":"journal-article","created":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T05:32:01Z","timestamp":1685079121000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":45,"title":["Neurovascular Uncoupling Is Linked to Microcirculatory Dysfunction in Regions Outside the Ischemic Core Following Ischemic Stroke"],"prefix":"10.1161","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5431-4409","authenticated-orcid":false,"given":"Christian","family":"Staehr","sequence":"first","affiliation":[{"name":"Aarhus University","place":["Aarhus, Denmark"]},{"name":"Boston University","place":["Boston, 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