{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T23:32:30Z","timestamp":1763767950114,"version":"3.41.2"},"reference-count":36,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Gene therapy has been proposed as a strategy to induce cardiac regeneration following acute myocardial infarction (AMI). Given that Tbx20, a transcription factor of the T\u2010box subfamily, stimulates cell proliferation and angiogenesis, we designed a baculovirus overexpressing\n              <jats:italic>Tbx20<\/jats:italic>\n              (Bv\u2010Tbx20) and evaluated its effects in cultured cardiomyocytes and in an ovine model of AMI.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Cell proliferation and angiogenesis were measured in cardiomyocytes transduced with Bv\u2010Tbx20 or Bv\u2010Null (control). Subsequently, in sheep with AMI, Bv\u2010Tbx20 or Bv\u2010Null was injected in the infarct border. Cardiomyocyte cell cycle activity, angioarteriogenesis, left ventricular function, and infarct size were assessed. Cardiomyocytes transduced with BvTbx20 increased cell proliferation, cell cycle regulatory and angiogenic gene expression, and tubulogenesis. At 7\u2009days posttreatment, sheep treated with Bv\u2010Tbx20 showed increased\n              <jats:italic>Tbx20<\/jats:italic>\n              , promitotic and angiogenic gene expression, decreased levels of\n              <jats:italic>P21<\/jats:italic>\n              , increased Ki67\u2010 (17.09\u00b15.73 versus 7.77\u00b17.24\u2009cardiomyocytes\/mm\n              <jats:sup>2<\/jats:sup>\n              ,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05) and PHH3 (phospho\u2010histone H3)\u2010labeled cardiomyocytes (10.10\u00b13.51 versus 5.23\u00b12.87\u2009cardiomyocytes\/mm\n              <jats:sup>2<\/jats:sup>\n              ,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05), and increased capillary (2302.68\u00b1353.58 versus 1694.52\u00b1211.36\u2009capillaries\/mm\n              <jats:sup>2<\/jats:sup>\n              ,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001) and arteriolar (146.95\u00b153.14 versus 84.06\u00b116.84\u2009arterioles\/mm\n              <jats:sup>2<\/jats:sup>\n              ,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05) densities. At 30\u2009days, Bv\u2010Tbx20 decreased infarct size (9.89\u00b11.92% versus 12.62\u00b11.33%,\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05) and slightly improved left ventricular function. Baculoviral gene transfer\u2010mediated Tbx20 overexpression exerted angiogenic and cardiomyogenic effects in\u00a0vitro.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">In sheep with AMI, Bv\u2010Tbx20 induced angioarteriogenesis, cardiomyocyte cell cycle activity, infarct size limitation, and a slight recovery of left ventricular function, suggesting that Bv\u2010Tbx20 gene therapy may contribute to cardiac regeneration following AMI.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.123.031515","type":"journal-article","created":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T10:38:03Z","timestamp":1721385483000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Intramyocardial Administration of Baculovirus Encoding the Transcription Factor Tbx20 in Sheep With Experimental Acute Myocardial Infarction"],"prefix":"10.1161","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1315-9630","authenticated-orcid":false,"given":"Mar\u00eda del Rosario","family":"Bauz\u00e1","sequence":"first","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4256-4683","authenticated-orcid":false,"given":"Ayel\u00e9n Emilce","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2707-5478","authenticated-orcid":false,"given":"Jorge Alejandro","family":"Simonin","sequence":"additional","affiliation":[{"name":"Instituto de Microbiolog\u00eda B\u00e1sica y Aplicada, Universidad Nacional de Quilmes","place":["Bernal, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2245-5148","authenticated-orcid":false,"given":"Francisco Stefano","family":"Cimbaro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agustina","family":"Scharn","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Araceli","family":"Castro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2003-9593","authenticated-orcid":false,"given":"Cintia Virginia","family":"Silvestro","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda (IMETTYB)\u2010Universidad Favaloro\u2010 CONICET","place":["Buenos Aires, Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5960-4333","authenticated-orcid":false,"given":"Luis Alberto","family":"Cuniberti","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Traslacional, Trasplante y Bioingenier\u00eda 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Reference values for echocardiographic parameters and indexes of left ventricular function in healthy, young adult sheep used in translational research: comparison with standardized values in humans. 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