{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:42:37Z","timestamp":1790376157072,"version":"4.1.0"},"reference-count":59,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,5,7]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">The purpose of this study was to investigate a therapeutic approach targeting the inflammatory response and consequent remodeling from ischemic myocardial injury.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Coronary thrombus aspirates were collected from patients at the time of ST\u2010segment\u2013elevation myocardial infarction and subjected to array\u2010based proteome analysis. Clinically indistinguishable at myocardial infarction (MI), patients were stratified into vulnerable and resilient on the basis of 1\u2010year left ventricular ejection fraction and death. Network analysis from coronary aspirates revealed prioritization of tumor necrosis factor\u2010\u03b1 signaling in patients with worse clinical outcomes. Infliximab, a tumor necrosis factor\u2010\u03b1 inhibitor, was infused intravenously at reperfusion in a porcine MI model to assess whether infliximab\u2010mediated immune modulation impacts post\u2010MI injury. At 3\u2009days after MI (n=7), infliximab infusion increased proregenerative M2 macrophages in the myocardial border zone as quantified by immunofluorescence (24.1%\u00b123.3% in infliximab versus 9.29%\u00b18.7% in sham;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.01). Concomitantly, immunoassays of coronary sinus samples quantified lower troponin I levels (41.72\u00b17.34\u2009pg\/mL versus 58.11\u00b110.75\u2009pg\/mL;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05) and secreted protein analysis revealed upregulation of injury\u2010modifying interleukin\u20102, \u20104, \u201010, \u201012, and \u201018 cytokines in the infliximab\u2010treated cohort. At 4\u2009weeks (n=12), infliximab treatment resulted in significant protective influence, improving left ventricular ejection fraction (53.9%\u00b15.4% versus 36.2%\u00b15.3%;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001) and reducing scar size (8.31%\u00b110.9% versus 17.41%\u00b112.5%;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Profiling of coronary thrombus aspirates in patients with ST\u2010segment\u2013elevation MI revealed highest association for tumor necrosis factor\u2010\u03b1 in injury risk. Infliximab\u2010mediated immune modulation offers an actionable pathway to alter MI\u2010induced inflammatory response, preserving contractility and limiting adverse structural remodeling.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.123.032172","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T10:21:03Z","timestamp":1714731663000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["Infliximab Limits Injury in Myocardial Infarction"],"prefix":"10.1161","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1772-450X","authenticated-orcid":false,"given":"Christopher","family":"Livia","sequence":"first","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Mayo Clinic Alix School of Medicine Mayo Clinic Graduate School of Biomedical Sciences Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3200-3575","authenticated-orcid":false,"given":"Sara","family":"Inglis","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Department of Cardiovascular Medicine Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9202-2922","authenticated-orcid":false,"given":"Ruben","family":"Crespo\u2010Diaz","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Department of Cardiovascular Medicine Mayo Clinic Rochester MN USA"},{"name":"Cardiovascular Division University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3754-7512","authenticated-orcid":false,"given":"Skylar","family":"Rizzo","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Mayo Clinic Alix School of Medicine Mayo Clinic Graduate School of Biomedical Sciences Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6126-1632","authenticated-orcid":false,"given":"Ryan","family":"Mahlberg","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Department of Cardiovascular Medicine Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4971-1180","authenticated-orcid":false,"given":"Monique","family":"Bagwell","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Mayo Clinic Alix School of Medicine Mayo Clinic Graduate School of Biomedical Sciences Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7115-7513","authenticated-orcid":false,"given":"Matthew","family":"Hillestad","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Department of Cardiovascular Medicine Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3072-4539","authenticated-orcid":false,"given":"Satsuki","family":"Yamada","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"},{"name":"Marriott Heart Disease Research Program Mayo Clinic Rochester MN USA"},{"name":"Department of Cardiovascular Medicine Mayo Clinic Rochester MN USA"},{"name":"Division of Geriatric &amp; Gerontology Medicine Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6251-6147","authenticated-orcid":false,"given":"Dhivya Vadhana","family":"Meenakshi Siddharthan","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1441-2722","authenticated-orcid":false,"given":"Raman Deep","family":"Singh","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Li","sequence":"additional","affiliation":[{"name":"Van Cleve Cardiac Regenerative Medicine Program Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2934-3782","authenticated-orcid":false,"given":"D. 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