{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T08:30:05Z","timestamp":1786869005235,"version":"build-2736575974"},"reference-count":31,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"18","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,9,17]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Diagnosis of the cause of cerebral thrombi is vital for recurrence prevention but also challenging. The presence of the microbiome has recently been confirmed in thrombus, suggesting a novel approach to distinguish cerebral thrombi of different origins. However, little is known about whether there is heterogeneity in microbiological colonization of cerebral thrombi of different sources.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Forty patients experiencing acute ischemic stroke were included and clinical data were collected. Metagenomic next\u2010generation sequencing was adopted to detect bacterial and genomic signatures of human cerebral thrombi samples. We found similar species diversity between the large\u2010artery atherosclerosis thrombi and cardioembolic thrombi but different species composition and distribution of cerebral thrombus microbiota. Compared with the group with cardioembolism, the group with large\u2010artery atherosclerosis showed a significantly higher relative abundance of\n              <jats:italic>Ralstonia insidiosa<\/jats:italic>\n              among the top 10 bacterial species in cerebral thrombi. Twenty operational taxonomy units were correlated with 11 clinical indicators of ischemic stroke. The Gene Ontology enrichment analysis revealed 9 different enriched biological processes (translation and carbohydrate metabolic process, etc). The enriched Kyoto Encyclopedia of Genes and Genomes pathways included ribosome, butanoate metabolism, and sulfur metabolism.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">This study, based on the approach of metagenomic next\u2010generation sequencing, provides a diagnostic microbiological method to discriminate individuals with cardioembolic thrombi from those with large\u2010artery atherosclerosis thrombi with human cerebral thrombi samples. Our findings provide a fresh perspective on microbial heterogeneity of cerebral thrombi and demonstrate biological processes and pathway features of cerebral thrombi.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.123.033221","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T11:18:18Z","timestamp":1725880698000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Microbial Heterogeneity Identification of Cerebral Thrombi Via Metagenomic Next\u2010Generation Sequencing\u2010Based Strategy"],"prefix":"10.1161","volume":"13","author":[{"given":"Xi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Neurology Southwest Hospital, Third Military Medical University (Army Medical University) Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of 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