{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T10:41:03Z","timestamp":1780051263388,"version":"3.53.1"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,4,16]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Cardiac donation after circulatory death is a promising option to increase graft availability. Graft preservation with 30\u2009minutes of hypothermic oxygenated perfusion (HOPE) before normothermic machine perfusion may improve cardiac recovery as compared with cold static storage, the current clinical standard. We investigated the role of preserved nitric oxide synthase activity during HOPE on its beneficial effects.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Using a rat model of donation after circulatory death, hearts underwent in\u00a0situ ischemia (21\u2009minutes), were explanted for a cold storage period (30\u2009minutes), and then reperfused under normothermic conditions (60\u2009minutes) with left ventricular loading. Three cold storage conditions were compared: cold static storage, HOPE, and HOPE with N\u03c9\u2010nitro\u2010L\u2010arginine methyl ester (nitric oxide synthase inhibitor). To evaluate potential confounding effects of high coronary flow during early reperfusion in HOPE hearts, bradykinin was administered to normalize coronary flow to HOPE levels in 2 additional groups (cold static storage and HOPE with N\u03c9\u2010nitro\u2010L\u2010arginine methyl ester). Cardiac recovery was significantly improved in HOPE versus cold static storage hearts, as determined by cardiac output, left ventricular work, contraction and relaxation rates, and coronary flow (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05). Furthermore, HOPE attenuated postreperfusion calcium overload. Strikingly, the addition of N\u03c9\u2010nitro\u2010L\u2010arginine methyl ester during HOPE largely abolished its beneficial effects, even when early reperfusion coronary flow was normalized to HOPE levels.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">HOPE provides superior preservation of ventricular and vascular function compared with the current clinical standard. Importantly, HOPE's beneficial effects require preservation of nitric oxide synthase activity during the cold storage. Therefore, the application of HOPE before normothermic machine perfusion is a promising approach to optimize graft recovery in donation after circulatory death cardiac grafts.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.123.033503","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T11:01:56Z","timestamp":1712919716000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Hypothermic Oxygenated Perfusion Improves Vascular and Contractile Function by Preserving Endothelial Nitric Oxide Production in Cardiac Grafts Obtained With Donation After Circulatory Death"],"prefix":"10.1161","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6368-7397","authenticated-orcid":false,"given":"Manuel","family":"Egle","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery Inselspital, Bern University Hospital, University of Bern 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Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8708-4996","authenticated-orcid":false,"given":"Selianne","family":"Graf","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Inselspital, Bern University Hospital, University of Bern Switzerland"},{"name":"Department for BioMedical Research University of Bern Switzerland"},{"name":"Graduate School for Cellular and Biomedical Sciences University of Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3305-9343","authenticated-orcid":false,"given":"Matthias","family":"Siepe","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Inselspital, Bern University Hospital, University of Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8489-917X","authenticated-orcid":false,"given":"Sarah","family":"Longnus","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Inselspital, Bern University Hospital, University of Bern Switzerland"},{"name":"Department for BioMedical Research University of Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehab368"},{"key":"e_1_3_2_3_2","unstructured":"Eurotransplant International Foundation . 2022 Annual Report. 2023. 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