{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:04:57Z","timestamp":1792821897283,"version":"4.5.0"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,5,7]]},"abstract":"<jats:sec sec-type=\"background\" xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Cardiac damage induced by ischemic stroke, such as arrhythmia, cardiac dysfunction, and even cardiac arrest, is referred to as cerebral\u2010cardiac syndrome (CCS). Cardiac macrophages are reported to be closely associated with stroke\u2010induced cardiac damage. However, the role of macrophage subsets in CCS is still unclear due to their heterogeneity. Sympathetic nerves play a significant role in regulating macrophages in cardiovascular disease. However, the role of macrophage subsets and sympathetic nerves in CCS is still unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              In this study, a middle cerebral artery occlusion mouse model was used to simulate ischemic stroke. ECG and echocardiography were used to assess cardiac function. We used\n              <jats:italic>Cx3cr1<\/jats:italic>\n              <jats:sup>\n                <jats:italic>GFP<\/jats:italic>\n              <\/jats:sup>\n              <jats:italic>Ccr2<\/jats:italic>\n              <jats:sup>\n                <jats:italic>RFP<\/jats:italic>\n              <\/jats:sup>\n              mice and\n              <jats:italic>NLRP3<\/jats:italic>\n              \u2010deficient mice in combination with Smart\u2010seq2 RNA sequencing to confirm the role of macrophage subsets in CCS. We demonstrated that ischemic stroke\u2010induced cardiac damage is characterized by severe cardiac dysfunction and robust infiltration of monocyte\u2010derived macrophages into the heart. Subsequently, we identified that cardiac monocyte\u2010derived macrophages displayed a proinflammatory profile. We also observed that cardiac dysfunction was rescued in ischemic stroke mice by blocking macrophage infiltration using a CCR2 antagonist and\n              <jats:italic>NLRP3<\/jats:italic>\n              \u2010deficient mice. In addition, a cardiac sympathetic nerve retrograde tracer and a sympathectomy method were used to explore the relationship between sympathetic nerves and cardiac macrophages. We found that cardiac sympathetic nerves are significantly activated after ischemic stroke, which contributes to the infiltration of monocyte\u2010derived macrophages and subsequent cardiac dysfunction.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">Our findings suggest a potential pathogenesis of CCS involving the cardiac sympathetic nerve\u2013monocyte\u2010derived macrophage axis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.123.034731","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T10:20:58Z","timestamp":1714731658000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Monocyte\u2010Derived Macrophages Aggravate Cardiac Dysfunction After Ischemic Stroke in Mice"],"prefix":"10.1161","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5269-3393","authenticated-orcid":false,"given":"Hong\u2010Bin","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9176-3585","authenticated-orcid":false,"given":"Pu","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0882-3901","authenticated-orcid":false,"given":"Meng\u2010Yu","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Jun","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4286-6374","authenticated-orcid":false,"given":"Jin\u2010Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center Sun Yat\u2010sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Pin","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan\u2010Xuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2613-0036","authenticated-orcid":false,"given":"Shi\u2010Yuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-1468","authenticated-orcid":false,"given":"Feng\u2010Xian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7558-4934","authenticated-orcid":false,"given":"Hong\u2010Fei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Zhujiang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.737170"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311170"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.2010.209304"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.118.022207"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.113.001714"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12264-020-00544-0"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.678995"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X18813317"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2022.08.009"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.314028"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.319737"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.11.019"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0059-x"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2017.12.006"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.303577"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.156236"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25073"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2009.06.024"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00209.2012"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042718-041812"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4422"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-500X.119726"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2023.114418"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacep.2021.12.014"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.7150\/thno.64033"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-16-2310"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.975367"},{"key":"e_1_3_1_30_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-210426"},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-020-00836-6"},{"key":"e_1_3_1_32_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000001601"},{"key":"e_1_3_1_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13258"},{"key":"e_1_3_1_34_2","doi-asserted-by":"publisher","DOI":"10.1261\/rna.053272.115"},{"key":"e_1_3_1_35_2","doi-asserted-by":"publisher","DOI":"10.3791\/51566-v"},{"key":"e_1_3_1_36_2","doi-asserted-by":"publisher","DOI":"10.2174\/1876386300902010011"},{"key":"e_1_3_1_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70291-5"},{"key":"e_1_3_1_38_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.122.026528"},{"key":"e_1_3_1_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30336-3"},{"key":"e_1_3_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjms.2015.10.020"},{"key":"e_1_3_1_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2018.10.002"},{"key":"e_1_3_1_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.07.003"},{"key":"e_1_3_1_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-022-00715-5"},{"key":"e_1_3_1_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10759"},{"key":"e_1_3_1_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-021-00762-2"},{"key":"e_1_3_1_46_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-111020-105359"},{"key":"e_1_3_1_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-023-01656-1"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.123.034731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T12:16:01Z","timestamp":1715602561000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.123.034731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,7]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,5,7]]}},"alternative-id":["10.1161\/JAHA.123.034731"],"URL":"https:\/\/doi.org\/10.1161\/jaha.123.034731","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,7]]},"assertion":[{"value":"2024-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e034731"}}