{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T11:26:53Z","timestamp":1790767613689,"version":"4.1.0"},"reference-count":65,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"14","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2024,7,16]]},"abstract":"<jats:sec id=\"jah39790-sec-0001\" xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Aging\u2010associated left ventricular dysfunction promotes cardiopulmonary fibrogenic remodeling, Group 2 pulmonary hypertension (PH), and right ventricular failure. At the time of diagnosis, cardiac function has declined, and cardiopulmonary fibrosis has often developed. Here, we sought to develop a molecular positron emission tomography (PET)\u2013magnetic resonance imaging (MRI) protocol to detect both cardiopulmonary fibrosis and fibrotic disease activity in a left ventricular dysfunction model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"jah39790-sec-0002\" xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      Left ventricular dysfunction was induced by transverse aortic constriction (TAC) in 6\u2010month\u2010old senescence\u2010accelerated prone mice, a subset of mice that received sham surgery. Three weeks after surgery, mice underwent simultaneous PET\u2010MRI at 4.7 T. Collagen\u2010targeted PET and fibrogenesis magnetic resonance (MR) probes were intravenously administered. PET signal was computed as myocardium\u2010 or lung\u2010to\u2010muscle ratio. Percent signal intensity increase and \u0394 lung\u2010to\u2010muscle ratio were computed from the pre\u2010\/postinjection magnetic resonance images. Elevated allysine in the heart (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.02) and lungs (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.17) of TAC mice corresponded to an increase in myocardial magnetic resonance imaging percent signal intensity increase (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.0001) and \u0394lung\u2010to\u2010muscle ratio (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.0001). Hydroxyproline in the heart (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.0001) and lungs (\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.01) were elevated in TAC mice, which corresponded to an increase in heart (myocardium\u2010to\u2010muscle ratio,\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      =0.02) and lung (lung\u2010to\u2010muscle ratio,\n                      <jats:italic toggle=\"no\">P<\/jats:italic>\n                      &lt;0.001) PET measurements. Pressure\u2010volume loop and echocardiography demonstrated adverse left ventricular remodeling, function, and increased right ventricular systolic pressure in TAC mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"jah39790-sec-0003\" xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Administration of collagen\u2010targeted PET and allysine\u2010targeted MR probes led to elevated PET\u2013magnetic resonance imaging signals in the myocardium and lungs of TAC mice. The study demonstrates the potential to detect fibrosis and fibrogenesis in cardiopulmonary disease through a dual molecular PET\u2013magnetic resonance imaging protocol.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.124.034363","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T07:55:44Z","timestamp":1720511744000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Simultaneous Positron Emission Tomography and Molecular Magnetic Resonance Imaging of Cardiopulmonary Fibrosis in a Mouse Model of Left Ventricular Dysfunction"],"prefix":"10.1161","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0642-0551","authenticated-orcid":false,"given":"Brianna F.","family":"Moon","sequence":"first","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4351-7398","authenticated-orcid":false,"given":"Iris Y.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2942-2202","authenticated-orcid":false,"given":"Yingying","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9916-2207","authenticated-orcid":false,"given":"Yin\u2010Ching I.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8680-0903","authenticated-orcid":false,"given":"Mariane","family":"Le Fur","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7903-0648","authenticated-orcid":false,"given":"Sergey","family":"Shuvaev","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2544-5762","authenticated-orcid":false,"given":"Eman A.","family":"Akam","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Cardiology, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7966-6862","authenticated-orcid":false,"given":"Hua","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0879-4058","authenticated-orcid":false,"given":"Cesar Molinos","family":"Solsona","sequence":"additional","affiliation":[{"name":"Bruker BioSpin, Preclinical Imaging","place":["Billerica, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6647-9769","authenticated-orcid":false,"given":"Jonah","family":"Weigand\u2010Whittier","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Rotile","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6190-624X","authenticated-orcid":false,"given":"Lida P.","family":"Hariri","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9669-4489","authenticated-orcid":false,"given":"Matthew","family":"Drummond","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2722-2524","authenticated-orcid":false,"given":"Avery T.","family":"Boice","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha E.","family":"Zygmont","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamini","family":"Sharma","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care and Sleep Medicine, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-8722","authenticated-orcid":false,"given":"Rod R.","family":"Warburton","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care and Sleep Medicine, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory L.","family":"Martin","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Research Institute, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6837-2076","authenticated-orcid":false,"given":"Robert M.","family":"Blanton","sequence":"additional","affiliation":[{"name":"Molecular Cardiology Research Institute, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry L.","family":"Fanburg","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care and Sleep Medicine, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8242-8339","authenticated-orcid":false,"given":"Nicholas S.","family":"Hill","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care and Sleep Medicine, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3179-6537","authenticated-orcid":false,"given":"Peter","family":"Caravan","sequence":"additional","affiliation":[{"name":"Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital and Harvard Medical School","place":["Boston, USA"]},{"name":"Institute for Innovation in Imaging, Massachusetts General Hospital","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2962-7758","authenticated-orcid":false,"given":"Krishna C.","family":"Penumatsa","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care and Sleep Medicine, Tufts Medical Center","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2012.06.002"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.10.033"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-60-15095"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.122.029024"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.632208"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv512"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.12316"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.111.186072"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2016.10.006"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.113.000887"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1183\/09059180.00003912"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2018180603"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.27849"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.121.025143"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.035326"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ehf2.12565"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2014.12.028"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1113\/JP275858"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf4696"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.117.193532"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070802"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.21037\/cdt-20-477"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.311148"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201903-0503LE"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.122.264530"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.91506"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201704773"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1039\/c9sc04821a"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abq6297"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.2c06231"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.0000000000000148"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2020192118"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85679-4"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c04964"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-42209-7"},{"key":"e_1_3_2_37_2","volume-title":"Guide for the Care and Use of Laboratory Animals","year":"2011","unstructured":"Guide for the Care and Use of Laboratory Animals, 8th edition. Washington (DC): National Academies Press (US); 2011."},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.060"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1162\/15353500200303133"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00987.2016"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-019-1100-3"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902155R"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.148003"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1570-0232(03)00248-4"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.08.032"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-020-0363-2"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.06.053"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318159"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00238.2018"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.560019"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2013-0039OC"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200700700"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2473070975"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2015.04.029"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2012.04.035"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.06.026"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108438"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202201-0142OC"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-022-02241-0"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.100.19.1992"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200011163432003"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.218.1.r01ja50215"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12968-016-0308-4"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1186\/1532-429X-15-92"},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00723.2004"},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00825.2009"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.124.034363","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T05:50:10Z","timestamp":1747288210000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.124.034363"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,16]]},"references-count":65,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2024,7,16]]}},"alternative-id":["10.1161\/JAHA.124.034363"],"URL":"https:\/\/doi.org\/10.1161\/jaha.124.034363","relation":{"is-referenced-by":[{"id-type":"doi","id":"10.1007\/s00259-025-07741-x","asserted-by":"object"}]},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,16]]},"assertion":[{"value":"2024-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e034363"}}