{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T11:10:09Z","timestamp":1784113809483,"version":"3.55.0"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2025,5,6]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Although e\u2010cigarettes provide an alternative to conventional smoking, the cardiovascular impacts of e\u2010cigarette use are unresolved. The popularity of menthol e\u2010cigarettes has surged recently and may escalate further with bans on combustible menthol cigarettes and e\u2010cigarette flavors other than menthol and tobacco. Despite recent evidence in mice that menthol e\u2010cigarettes acutely induce cardiac arrhythmias, the impacts of repeated menthol e\u2010cigarette use on cardiovascular function and the cardiac proteome remain unclear. We therefore investigated the acute and persistent cardiovascular effects of menthol e\u2010cigarettes in a mouse model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">Adult C57BL\/6J mice with ECG and blood pressure radiotransmitters were exposed to e\u2010cigarette aerosols (180\u2013270\u2009puffs\/day; n=4\u20138\/group). One\u2010day exposures to nicotine\u2010containing e\u2010cigarette aerosols depressed heart rate variability regardless of flavor, but menthol e\u2010cigarette aerosols uniquely increased heart rate and urine epinephrine and elicited spontaneous ventricular premature beats. Menthol e\u2010cigarette aerosols consistently increased blood pressure acutely, and this effect recurred throughout the 20\u2010day regimen. Pretreatment with atenolol abolished e\u2010cigarette\u2013induced arrhythmias, suggesting the involvement of \u03b21\u2010adrenoceptors. After 4\u2009weeks of exposure to JUUL Menthol aerosol, mice had basal sinus bradycardia that persisted up to 3\u2009weeks after exposure cessation. After cessation, e\u2010cigarette\u2013exposed mice also exhibited an altered chronotropic response to restraint stress and prolonged ventricular repolarization (corrected QT interval). Integrated proteomic and phosphoproteomic analysis of cardiac tissue harvested from mice exposed to menthol e\u2010cigarette aerosols for 5 and 20\u2009days revealed molecular signatures of dilated and arrhythmogenic cardiomyopathy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Exposure to menthol e\u2010cigarette aerosols induces persistent cardiovascular autonomic imbalance in\u00a0vivo. These findings raise the possibility of similar effects in humans using mentholated e\u2010cigarettes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.124.037420","type":"journal-article","created":{"date-parts":[[2025,4,26]],"date-time":"2025-04-26T00:01:02Z","timestamp":1745625662000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Acute and Persistent Cardiovascular Effects of Menthol E\u2010Cigarettes in Mice"],"prefix":"10.1161","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1800-1020","authenticated-orcid":false,"given":"Anand R.","family":"Ramalingam","sequence":"first","affiliation":[{"name":"University of Louisville","place":["USA"]},{"name":"American Heart Association Tobacco Regulation and Addiction Center","place":["Dallas, USA"]},{"name":"University of 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