{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:17:51Z","timestamp":1788383871257,"version":"build-2803163510"},"reference-count":77,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:sec xml:lang=\"en\">\n                    <jats:title>Background<\/jats:title>\n                    <jats:p xml:lang=\"en\">Chronic arterial hypertension restructures the vascular architecture of the brain, leading to a series of pathological responses that culminate in cerebral small\u2010vessel disease. Pericytes respond dynamically to vascular challenges; however, how they manifest under the continuous strain of hypertension has not been elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p xml:lang=\"en\">\n                      In this study, we characterized pericyte behavior alongside hypertensive states in the spontaneously hypertensive stroke\u2010prone rat model, focusing on their phenotypic and metabolic transformation. Flow cytometry was used to characterize pericytes by their expression of platelet\u2010derived growth factor receptor \u03b2, neuroglial antigen 2, cluster of differentiation 13\u2013alanyl aminopeptidase, and antigen Kiel 67. Microvessels were isolated for gene expression profiling and in\u00a0vitro pericyte expansion. Immunofluorescence validated the cell culture model. Plasma\u2010derived extracellular vesicles from hypertensive rodents were applied as a treatment to assess their effects on pericyte function and detailed metabolic assessments on enriched pericytes measured oxidative phosphorylation and glycolysis. Our results reveal a shift in platelet\u2010derived growth factor receptor \u03b2\n                      <jats:sup>+<\/jats:sup>\n                      pericytes toward increased neuroglial antigen 2 and cluster of differentiation 13\u2013alanyl aminopeptidase coexpression, indicative of their critical role in vascular stabilization and inflammatory responses within the hypertensive milieu. Significant alterations were found within key pathways including angiogenesis, blood\u2013brain barrier integrity, hypoxia, and inflammation. Circulating extracellular vesicles from hypertensive rodents distinctly influenced pericyte mitochondrial function, evidencing their dual role as carriers of disease pathology and potential therapeutic agents. Furthermore, a shift toward glycolytic metabolism in hypertensive pericytes was confirmed, coupled with ATP production dysregulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec xml:lang=\"en\">\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p xml:lang=\"en\">Our findings demonstrate that cerebral pericytes undergo phenotypic and metabolic reprogramming in response to hypertension, with hypertensive\u2010derived plasma\u2010derived extracellular vesicles impairing their mitochondrial function. Importantly, plasma\u2010derived extracellular vesicles from normotensive controls restore this function, suggesting their potential as both therapeutic agents and precision biomarkers for hypertensive vascular complications. Further investigation into plasma\u2010derived extracellular vesicle cargo is essential to further explore their therapeutic potential in vascular health.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/jaha.124.038457","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T22:57:26Z","timestamp":1735081046000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Pericytes and Extracellular Vesicle Interactions in Neurovascular Adaptation to Chronic Arterial Hypertension"],"prefix":"10.1161","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3957-3848","authenticated-orcid":false,"given":"Lorena","family":"Morton","sequence":"first","affiliation":[{"name":"Medical Faculty, Institute of Inflammation and Neurodegeneration Otto\u2010von\u2010Guericke University Magdeburg Magdeburg 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